Abstract
The establishment of the steady-state rate of photosynthetic O2 evolution by cells of Anabaena variabilis and other cyanobacteria was found to be preceded by a lag-phase the duration of which depended on the time of cell preincubation in the dark. Electron acceptors (benzoquinone, N,N,N′,N′-tetramethyl-p-phenylenediamine, 2,3,5,6-tetramethyl-p-phenylenediamine or 2,6-dichlorophenolindophenol) abolished the lag-phase as well as the inhibitory effect of cyanide on electron transfer. Mono-, di-and trivalent cations added to the cell suspension markedly reduced the lag-phase. As cation concentrations were increased, acceleration and subsequent deceleration of the O2 evolution rate were observed. The efficient concentrations of cations decreased as their valency increased. The lag-phase and the rate of photosynthetic O2 evolution by the blue-green algae are suggested to depend on the value of the membrane surface charge governing the electrostatic interaction between unidentified membrane-bound redox components. The combination of valinomycin and nigericin as well as gramicidin D enhanced the duration of the lagphase by deenergization of thylakoid membrane.
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Abbreviations
- 9AA:
-
9-aminoacridine
- BQ:
-
benzoquinone
- DAD:
-
2,3,5,6-tetramethyl-p-phenylenediamine
- DPIP:
-
2,6-dichlorophenolindophenol
- FeCy:
-
ferrycyanide
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethane-sulphonic acid
- MES:
-
2(N-morpholino)ethane sulphonic acid
- TMPD:
-
N,N,N′N′-tetramethyl-p-phenylenediamine
- Tris:
-
tris(hydroxymethyl)aminomethane
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Barsky, E.L., Gusev, M.V., Kazennova, N.V. et al. Surface charge on thylakoid membranes: regulation of photosynthetic electron transfer in cyanobacteria. Arch. Microbiol. 138, 54–57 (1984). https://doi.org/10.1007/BF00425407
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DOI: https://doi.org/10.1007/BF00425407