Summary
The influence of glucose on adenine nucleotide levels and energy charge in a growing culture of Acetobacter aceti and a glucose sensitive mutant, whose growth on ethanol is inhibited by glucose, was determined.
-
1.
Both strains showed an abrupt fall of the intracellular ATP after substrate exhaustion.
-
2.
Glucose alone could not maintain the energy charge in either strain at the high values observed during growth on ethanol.
-
3.
Glucose lowered the total adenine nucleotide content in the mutant in the absence of ethanol.
-
4.
During the inhibition of growth by glucose in the glucose sensitive mutant, three phases could be distinguished:
-
a)
A first period with a transient decline in the total adenine nucleotide level and no change in the energy charge.
-
b)
A recovery of the total adenine nucleotide level to its previous value. This second phase lasted as long as there was ethanol in the medium.
-
c)
A third period after ethanol exhaustion characterized by a lower total adenine nucleotide level in the presence of glucose and a lower energy charge.
-
a)
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atkinson, D. E., Walton, G. M.: Adenosine triphosphate conservation in metabolic regulation. Rat liver citrate cleavage enzyme. J. biol. Chem. 242, 3239–3241 (1967)
Benziman, M., Palgi, A.: Characterisation and properties of the pyruvate phosphorylation system of Acetobacter xylinum. J. Bact. 104, 211–218 (1970).
Chapman, A. G., Fall, L., Atkinson, D. E.: Adenylate energy charge in Escherichia coli during growth and starvation. J. Bact. 108, 1072–1086 (1971)
Harrison, D. E. F., Maitra, P. K.: Control of respiration and metabolism in growing Klebsiella aerogenes. The role of adenine nucleotides. Biochem. J. 112, 647–656 (1969)
Holms, W.H., Hamilton, I. D., Robertson, A. G.: The rate of turnover of the adenosine triphosphate pool of Escherichia coli growing aerobically in simple defined media. Arch. Mikcrobiol. 83, 95–109 (1972)
Holz, G., Bergmeyer, H. U.: Acetat, Bestimmung mit Acetatkinase und Hydroxylamin. In: H. U. Bergmeyer. Methoden der enzymatischen Analyse, S. 1486 bis 1490 Weinheim: Verlag Chemie 1970
Knowles, Ch. J., Smith, L.: Measurements of ATP levels of intact Azotobacter vinelandii under different conditions. Biochim. biophys. Acta (Amst.) 197, 152–160 (1970)
Lien, O. J., Jr.: Determination of gluconolactone, galactolactone and their free acids by the hydroxamate method Analyt. Chem. 31: 1363–1366 (1959).
Monard, D.: Contribution à l'étude du métabolisme d'Acetobacter aceti. Diss. No. 4172 ETHZ. Zürich: Juris 1968
Pradet, A.: Etude des adénosine-5′-mono, di- et tri-phosphates dans les tissus végétaux. I. Dosage enzymatique. Physiol. Vég. 5, 209–221 (1967)
Somlo, M.: Respiration-linked ATP formation by an “oxidative phosphorylation mutant” of yeast. Arch. Biochem. Biophys. 136, 122–133 (1970)
Strehler, B. L.: Adenosin-5′-triphosphat und Creatinphosphat. Bestimmung mit Luciferase. In: H. U. Bergmeyer: Methoden der enzymatischen Analyse, P. 2036–2045. Weinheim: Verlag Chemie 1970
Van der Beek, E. G., Stouthamer, A. H.: Oxidative phosphorylation in intact bacteria. Arch. Mikrobiol. 89, 327–339 (1973)
Weber, P.: Der Einfluß von Glucose auf den Äthanolstoffwechsel eines Stammes von Acetobacter aceti. Diss. No. 4794 ETHZ. Zürich: Juris 1972
Winkler, H. H. Wilson, T.H.: The role of energy coupling in the transport of β-galactosides by Escherichia coli. J. biol. Chem. 241, 220–221 (1966)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bächi, B., Ettlinger, L. Influence of glucose on adenine nucleotide levels and energy charge in Acetobacter aceti . Archiv. Mikrobiol. 93, 155–164 (1973). https://doi.org/10.1007/BF00424946
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00424946