Summary
Mutants of Aspergillus niger requiring adenine and one mutant requiring cytosine were isolated after low-dose mutagenesis and enrichment. In addition we had mutants of two genes involved in the pyrimidine biosynthesis isolated as 5-fluoro-orotic acid-resistant mutants. The fifteen adenine-less mutants could be placed in seven complementation groups. From each group a representative mutant was analyzed in order to determine the linkage group by analysis of the mutants in a heterozygous diploid carrying markers in six linkage groups. AdeF could not be assigned to any one of these linkage groups and proved to be linked to nicB, oliC and cnxC, none of which could be placed in a linkage group. Thus, conclusive evidence was obtained for a seventh linkage group. As pyrA was used as selection marker for transformation, we constructed a pyrA strain with a linked marker which can be used in the genetic analysis of transformants.
References
Ballance DJ, Turner G (1985) Gene 36:321–331
Bos CJ (1987) Curr Genet 12:471–474
Bos CJ, Debets AJM, Swart K, Huybers A, Kobus G, Slakhorst SM (1988) Curr Genet 14:437–44
Debets AJM, Swart K, Bos CJ (1989) Can J Microbiol (in press)
Goosen T, Bloemheuvel G, Gysler C, De Bie DA, Van den Broek HWJ, Swart K (1987) Curr Genet 11:499–503
Goosen T, Van Engelenburg F, Debets F, Swart K, Bos K, Van den Broek H (1989) Mol gen Genet (in press)
Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) Adv Genet 5:141–238
Van Hartingsveldt W, Mattern IE, Van Zeijl CMJ, Pouwels PH, Van den Hondel CAMJJ (1987) Mol Gen Genet 206:71–75
Wernars K (1986) PhD thesis. Agricultural University, Wageningen
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bos, C.J., Debets, A.J.M., Kobus, G. et al. Adenine and pyrimidine genes of Aspergillus niger and evidence for a seventh linkage group. Curr Genet 16, 307–310 (1989). https://doi.org/10.1007/BF00422118
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00422118