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The lipopolysaccharide of the phototrophic bacterium Ectothiorhodospira vacuolata

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Abstract

The lipopolysaccharide of Ectothiorhodospira vacuolata was obtained by the phenol-water procedure. It contained a 3-O-methyl-hexose, glucose, galacturonic and glucuronic acids. The finding of d-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate (tentatively identified) suggested a core-structure. The lipid fraction of the lipopolysaccharide contained phosphate and both, 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine. The major fatty acids were amine-bound 3-OH-10:0 and 3-OH-12:0 and esterbound 14:0 and 16:0 Sodium deoxycholate gel-electrophoresis, showing a single band only, indicated R-type character of the lipopolysaccharide of Ectothiorhodospira vacuolata.

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Abbreviations

DOC:

sodium deoxycholate

GC/MS:

combined gasliquid chromatography

PAGE:

polyacrylamide gel-electrophoresis

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Meißner, J., Borowiak, D., Fischer, U. et al. The lipopolysaccharide of the phototrophic bacterium Ectothiorhodospira vacuolata . Arch. Microbiol. 149, 245–248 (1988). https://doi.org/10.1007/BF00422012

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  • DOI: https://doi.org/10.1007/BF00422012

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