Abstract
The lipopolysaccharide of Ectothiorhodospira vacuolata was obtained by the phenol-water procedure. It contained a 3-O-methyl-hexose, glucose, galacturonic and glucuronic acids. The finding of d-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate (tentatively identified) suggested a core-structure. The lipid fraction of the lipopolysaccharide contained phosphate and both, 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine. The major fatty acids were amine-bound 3-OH-10:0 and 3-OH-12:0 and esterbound 14:0 and 16:0 Sodium deoxycholate gel-electrophoresis, showing a single band only, indicated R-type character of the lipopolysaccharide of Ectothiorhodospira vacuolata.
Similar content being viewed by others
Abbreviations
- DOC:
-
sodium deoxycholate
- GC/MS:
-
combined gasliquid chromatography
- PAGE:
-
polyacrylamide gel-electrophoresis
References
Blumenkrantz N, Asboe-Hansen G (1973) New Method for quantitative determination of uronic acids. Anal Biochem 54:484–489
Evers D, Weckesser J, Jürgens UJ (1986) Chemical analyses on cell envelope polymers of the halophilic, phototrophic Rhodospirillum salexigens. Arch Microbiol 145:254–258
Hurlbert RE, Weckesser J, Mayer H, Fromme I (1976) Isolation and characterization of the lipopolysaccharide of Chromatium vinosum. Eur J Biochem 68:365–371
Hurlbert RE, Hurlbert I (1977) Biological and physiochemical properties of the lipopolysaccharide of Chromatium vinosum. Infect Immun 16:983–994
Hurlbert RE, Weckesser J, Tharanathan RN, Mayer H (1978) Isolation and characterization of the lipopolysaccharide of Thiocapsa roseopersicina. Eur J Biochem 90:241–246
Imhoff JF (1984) Reassignment of the genus Ectothiorhodospira Pelsh 1936 to a new family. Ectothiorhodospiraceae fam. nov., and emended description of the Chromatiaceae Bavendamm 1924. Int J Syst Bacteriol 34:338–339
Imhoff JF, Tindall BJ, Grant WD, Trüper HG (1981) Ectothiorhodospira vacuolata sp. nov., a new phototrophic bacterium from soda lakes. Arch Microbiol 130:238–242
Jürgens UJ, Drews G, Weckesser J, (1983) Primary structure of the peptidoglycan from the unicellular cyanobacterium Synechocystis sp. strain PCC 6714. J Bacteriol 154:471–478
Kasai N, Arata S, Mashima JI, Akiyama Y, Tanaka C, Egawa K, Tanaka S (1987) Pseudomonas diminuta LPS with a new endotoxid lipid A structure. Biochem Biophys Res Commun 142:972–978
Komuro T, Galanos C (1986) Analysis of Salmonella lipopolysaccharides by sodium deoxycholate-polyacrylamide gel electrophoresis. EOS-Riv Immunol 6:147
Lowry OH, Roberts NR, Leiner KY, Wu ML, Farr AL (1954) The quantitative histochemistry of brain. J Biol Chem 207:1–17
Lüderitz O, Freudenberg MA, Galanos C, Lehmann V, Rietschel ET, Shaw DH (1982) Lipopolysaccharides of Gram-negative bacteria. Current Topics in Membranes and Transport 17:79–151
Mayer H, Weckesser J (1984) ‘Unusual’ lipid A's: structures, taxonomical relevance and potential value for endotoxin research. In: Rietschel ETh (ed) Handbook of endotoxin, vol 1: Chemistry of endotoxin. Elsevier Science Publishers, Amsterdam, pp 221–247
Niedermeier W, Tomana M (1974) Gas chromatographic analysis of hexosamines in glycoproteins. Anal Biochem 57:363–368
Roppel J, Mayer H, Weckesser J (1975) Identification of a 2,3-diamino-2,3-dideoxyhexose in the lipid A component of lipopolysaccharides of Rhodopseudomonas viridis and Rhodopseudomonas palustris. Carbohydr Res 40:31–40
Sleytr UB, Messner P (1983) Crystalline surface layers of bacteria. Annu Rev Microbiol 37:311–319
Snyder F, Stephens N (1959) A simplified spectrophotometric determination of ester groups in lipids. Biochim Biophys Acta 34:244–245
Stackebrandt E, Fowler VJ, Schubert W, Imhoff JF (1984) Towards a phylogeny of phototrophic purple sulfur bacteria —the genus Ectothiorhodospira. Arch Microbiol 137:336–370
Trüper HG, Imhoff JF (1981) The genus Ectothiorhodospira. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer Berlin Heidelberg New York, pp 274–278
Tsai CM, Frasch CE (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119:115–119
Warawdekar VS, Saslaw LD (1959) A sensitive method for the estimation of 2-deocy sugars with the use of the thiobarbituric acid reaction. J Biol Chem 234:1945–1950
Weckesser J, Mayer H (1987) Lipopolysaccharides of phototrophic bacteria, a contribution to phylogeny and endotoxin-research. Forum Mikrobiologie 7/8:242–248
Weckesser J, Drews G, Mayer H (1979) Lipopolysaccharides of photosynthetic prokaryotes. Ann Rev. Microbiol 33:215–239
Westphal O, Lüderitz O, Bister F (1952) Über die Exktraktion von Bakterien mit Phenol/Wasser. Z Naturforsch 7b:148–155
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meißner, J., Borowiak, D., Fischer, U. et al. The lipopolysaccharide of the phototrophic bacterium Ectothiorhodospira vacuolata . Arch. Microbiol. 149, 245–248 (1988). https://doi.org/10.1007/BF00422012
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00422012