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Comparison of RAPD and RFLP markers for mapping F2 generations in maize (Zea mays L.)

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Abstract

The F2 generations from two maize crosses were used to compare the ability of RAPD and RFLP marker systems to create a genetic linkage map. Both RFLPs and RAPDs were shown to provide Mendelian-type markers. Most of the RFLPs (80%) could be placed with a good level of certainty (LOD>4) on the genetic linkage map. However, because of their dominant nature, only between 37% and 59% of the RAPDs could be placed with such a LOD score. The use of combined data from RFLPs and RAPDs increases the level of information provided by RAPDs and allows the creation of a combined RFLP/RAPD genetic linkage map. Thus, the RAPD technique was found to be a powerful method to provide improved probes coverage on a previously created RFLP map and to locate markers linked to chromosomal regions of interest.

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Communicated by G. E. Hart

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Beaumont, V.H., Mantet, J., Rocheford, T.R. et al. Comparison of RAPD and RFLP markers for mapping F2 generations in maize (Zea mays L.). Theoret. Appl. Genetics 93, 606–612 (1996). https://doi.org/10.1007/BF00417955

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  • DOI: https://doi.org/10.1007/BF00417955

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