Abstract
Lipid A's from two Bradyrhizobium species and from the phylogenetically closely related species “Pseudomonas carboxydovorans” were found to contain 2,3-diamino-2,3-dideoxy-glucose as lipid A backbone sugar. In contrast, three representatives of the genus Rhizobium, as well as the phylogenetically related species Agrobacterium tumefaciens, contain solely glucosamine as lipid A backbone sugar. These findings suppor independent studies on the phylogenetical relatedness based on 16S rRNA-data of the genus Bradyrhizobium with “Pseudomonas carboxydovorans“ and Rhodopseudomonas palustris, which form a tight phylogenetical cluster and which all contain the 2,3-diamino-2,3-dideoxy-glucose-containing lipid A. The relatedness of these species to the glucosamine-containing species of the genus Rhizobium and to Agrobacterium tumefaciens is rather distant as documented by 16S rRNA studies.
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Abbreviations
- LPS:
-
lipopolysaccharide
- KDO:
-
2-keto-3-deoxyoctonic acid
- GalA:
-
galacturonic acid
- ld-heptose:
-
l-glycero-d-manno-heptose
- dd-heptose:
-
d-glycero-d-manno-heptose
- DOC:
-
sodium deoxycholate
- PAGE:
-
polyacrylamide gel electrophoresis
- DAG:
-
2,3-diamino-2,3-dideoxy-glucose
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Mayer, H., Krauss, J.H., Urbanik-Sypniewska, T. et al. Lipid A with 2,3-diamino-2,3-dideoxy-glucose in lipopolysaccharides from slow-growing members of Rhizobiaceae and from “Pseudomonas carboxydovorans”. Arch. Microbiol. 151, 111–116 (1989). https://doi.org/10.1007/BF00414423
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DOI: https://doi.org/10.1007/BF00414423