Summary
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1.
A water soluble cytochrome c from Hemispora stellata has been obtained in a highly purified state by a series of steps involving (NH4)2SO4 precipitation and DEAE chromatography.
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2.
Absorption spectrum of the highly purified cytochrome c (550; H. stellata) in the reduced state shows maxima at 550, 520, 416, 357–362 nm and 310–320 nm. It has a symmetrical α band and ratios of αred./prot.ox and Soretred/αred. are 1 and 6.4 respectively. The purified cytochrome has a mesoheme prosthetic group, its molecular weight is about 17500, its isoelectric point is at pH higher than 8.6 and its É o is+0.28.
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3.
During DEAE chromatography of buffer extracts as well as of purified samples of cytochrome c (550; H. stellata) two bands were formed. Chromatographic experiments performed with pure samples of cytochrome c (550; H. stellata) and with horse heart cytochrome c from a commercial source suggest that this second band in the DEAE chromatographies is due to protein denaturation during the purification procedure.
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4.
Cytochrome c (550; H. stellata) is similar to cytochromes of microorganisms belonging to the families Cryptococcaceae and Moniliaceae and although it is also spectrophotometrically similar to cytochrome c from horse heart it has a highar molecular weight and midpoint redox potential.
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Sala, J.F.T., Burgos, J. Purification and properties of cytochrome c (550; Hemispora stellata). Archiv. Mikrobiol. 88, 11–24 (1973). https://doi.org/10.1007/BF00408837
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DOI: https://doi.org/10.1007/BF00408837