Summary
The frequency of SCE was investigated in peripheral lymphocytes of one patient with XP. Although lymphocytes did not show high levels of spontaneous chromosome damage, 10-8 M Mitomycin C greatly increased the frequency of SCE. The results are interpreted as an expression of mutagen induced chromosomal instability due to impaired DNA repair, which is a significant anomaly of the disease.
Zusammenfassung
Obwohl die Lymphocyten eines Patienten mit XP keinen besonderen Hinweis auf eine spontane Chromosomenschädigung zeigen, hat 10-8 M Mitomycin C die Frequenz des SCE sehr erhöht. Dieses Ergebnis kann als Ausdruck einer von Mutagenen stimulierten — dem DNA repair zugeschriebenen — Chromosomenunbeständigkeit betrachtet werden.
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References
Arlett CF, Lehmann AR (1978) Human disorders showing increased sensitivity to the induction of genetic damage. Ann Rev Genet 12:95–115
Bootsma D (1977) Defective DNA repair and cancer. In: Castellani A (ed) Research in photobiology. Plenum Press, New York, pp 455–468
Burgdorf W, Kurvink K, Cervenka J (1977) Elevated sister chromatid exchange rate in lymphocytes of subjects treated with arsenic. Hum Genet 36:69–72
Changanti RSK, Schonberg S, German J (1974) A manifold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Proc Natl Acad Sci USA 71:4508–4512
Cleaver JE (1968) Defective repair replication of DNA in Xeroderma pigmentosum. Nature 218:652–656
Cleaver JE (1972) Xeroderma pigmentosum: variants with normal DNA repair and normal DNA repair and normal sensitivity to ultraviolet light. J Invest Dermatol 58:124–128
Galloway SM, Evans HJ (1975) Sister chromatid exchange in human chromosomes from normal individuals and patients with ataxia teleangiectasia. Cytogenet Cell Genet 15:17–29
Latt SA (1974) Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by Mitomycin C. Proc Natl Acad Sci USA 71:3162–3166
Perry P, Evans HJ (1975) Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature 218:121–125
Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158
Robbins JH, Kraemer HK, Lutzner MA, Festoff BW, Coon GH (1974) Xeroderma pigmentosum. Ann Intern Med 80:221–248
Went M, Kenderessy-Szabó A, Pólay A, Simon N (1981) Study of DNA repair of a xeroderma pigmentosum patient and his heterozygotic parents. Arch Dermatol Res 270:291–299
Wolff S, Rodin B, Cleaver JE (1977) Sister chromatid exchanges induced by mutagenic carcinogens in normal and xeroderma pigmentosum cells. Nature 265:347–349
Wolff S, Bodycote J, Thomas GH, Cleaver JE (1975) Sister chromatid exchange in xeroderma pigmentosum cells that are defective in DNA excision repair or post-replication repair. Genetics 81:349–355
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Went, M., Szőrenyi, Á., Polay, A. et al. Investigation of the sister chromatid exchange (SCE) frequency in one patient with xeroderma pigmentosum. Arch Dermatol Res 271, 407–409 (1981). https://doi.org/10.1007/BF00406685
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DOI: https://doi.org/10.1007/BF00406685