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Photoperiodic and osmotic influences on the ultrastructure of the hypothalamic neurosecretory system of the White-crowned sparrow, Zonotrichia leucophrys gambelii

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Summary

An attempt was made to correlate functional changes in the neurohypophysis of the White-crowned Sparrow, Zonotrichia leucophrys gambelii, with morphologic features on the light- and electron-microscope levels. The aldehyde-fuchsin-staining anterior median eminence possesses essentially the same ultrastructural features as the non-staining posterior median eminence. The axon terminals are characterized by the presence of a large number of small vesicles (approximately 400 Å in diameter) and occasional electron-dense granules. The more-or-less “depleted” anterior median eminence occasionally evident in the photosensitive bird showing testicular development is indistinguishable ultrastructurally from the more intensely staining median eminence generally characteristic of the photorefractory bird. In the median eminence, stainability and functional state do not seem to be correlated with changes in the type, size or number of vesicles. A slight increase in the number of granules was noted in the photorefractory bird but this was considered insufficient basis to account for differences in stainability.

The pars nervosa, on the other hand, responded to osmotic stimuli (saline drinking water) by loss of stainability and decrease in numbers of elementary neurosecretory granules. Small vesicles are also present in the pars nervosa axon terminals, but are intermingled with neurosecretory granules in normal birds. “Acute-osmotic” birds, however, had axon terminals almost entirely occupied by small vesicles.

It is to be emphasized that the pars nervosa and the median eminence are two structurally very different regions of the neurohypophysis. The basis for aldehyde-fuchsin staining in the median eminence appears to differ from that in the pars nervosa. The implications of these findings are considered in regard to hypothalamic control over gonadotropic activity in the White-crowned Sparrow.

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Authors and Affiliations

  1. Department of Zoology and its Cancer Research Genetics Laboratory, University of California, Berkeley

    Howard A. Bern, Richard S. Nishioka, L. Richard Mewaldt & Donald S. Farner

  2. Department of Biological Sciences, San Jose State College, USA

    Howard A. Bern, Richard S. Nishioka, L. Richard Mewaldt & Donald S. Farner

  3. Laboratories of Zoophysiology, Washington State University, Pullman, USA

    Howard A. Bern, Richard S. Nishioka, L. Richard Mewaldt & Donald S. Farner

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  1. Howard A. Bern
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  2. Richard S. Nishioka
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  3. L. Richard Mewaldt
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  4. Donald S. Farner
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Dedicated to Professor Dr. W. Bargmann in honor of his 60th birthday.

This investigation was supported by grant GB-2484 from the National Science Foundation to Professor Bern, grant GB-2819 from the National Science Foundation to Professor Mewaldt, and grant NB-01353 from the National Institutes of Health to Professor Farner. The authors wish to express their appreciation of the technical assistance of Mrs. Irene Brown, Mr. John Butchart, Sally S. Kibby, Mrs. Carol Nicoll, and Mr. John Striffler. Mrs. Emily Reid kindly prepared the histograms.

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Bern, H.A., Nishioka, R.S., Mewaldt, L.R. et al. Photoperiodic and osmotic influences on the ultrastructure of the hypothalamic neurosecretory system of the White-crowned sparrow, Zonotrichia leucophrys gambelii . Z.Zellforsch 69, 198–227 (1966). https://doi.org/10.1007/BF00406275

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