Abstract
The effect of a low phosphate concentration on intracellular adenine nucleotide content, oxygen consumption and poly-β-hydroxybutyrate deposition was investigated with N-free and NH +4 batch cultures of Azotobacter vinelandii. When the microorganisms were cultured under low-phosphate concentrations the cells contained much larger amounts of poly-β-hydroxybutyrate, but displayed lower oxygen consumption activities and energy charge values than did control cells. Also, the ratio ATP to ADP was much higher in control cells and the intracellular levels of ATP were lower in low-phosphate cells.
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Aladegbami SL, Tsai JC, Vela GR (1979) Adenilate energy charge of Azotobacter vinelandii during encystment. Curr Microbiol 2:327–329
Allen RJL (1940) Estimation of phosphorus. Biochem J 34:858–865
Ballesteros F, Gonzalez-Lopez J, de la Rubia T, Moreno J, Aneiros J, Ramos-Cormenzana A (1986) Growth of Azotobacter vinelandii in dialysed soil medium: Studies upon the life cycle. Ann Inst Pasteur Microbiol 137:55–64
Chapman AG, Atkinson DE (1977) Adenine nucleotide concentration and turnover rates. Their correlation with biological activity in bacteria and yeast. Adv Microbiol Phys 15:253–306
Chapman AG, Fall L, Atkinson DE (1971) Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol 108:1072–1086
Dalton H, Postgate JR (1969a) Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures. J Gen Microbiol 54:463–473
Dalton H, Postgate JR (1969b) Growth and physiology of Azotobacter chroococcum in continuous culture. J Gen Microbiol 56:307–319
Edwards SW, Lloyd D (1978) Oscillation of respiration and adenine nucleotides in synchronous cultures of Acanthamoeba castellani: mitochondrial respiration control in vivo. J Gen Microbiol 108:197–204
Forsberg GW, Wyrick PB, Ward JB, Rogers HJ (1973) Effect of phosphate-limitation on the morphology and cell wall composition of Bacillus licheniformis and its phosphoglucomutase-deficient mutants. J Bacteriol 113:969–984
Grant WD (1979) Cell wall teichoic acid as a reserve phosphate source in Bacillus subtilis. J Bacteriol 137:35–43
Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in Microbiology, vol 5B. Academic Press, London, pp 209–344
Law JH, Slepecky RA (1961) Assay of poly-β-hydroxybutyric acid. J Bacteriol 82:33–36
Marriot ID, Dawes EA, Rowley BI (1981) Effect of growth rate and nutrient limitation on the adenine nucleotide content, energy charge and enzymes of adenylate metabolism in Azotobacter beijerinckii. J Gen Microbiol 125:375–382
Martinez-Toledo MV, Gonzalez-Lopez J, Salmeron V, de la Rubia T, Ballesteros F, Ramos-Cormenzana A (1986) Properties of Azotobacter vinelandii in phosphate-limited batch cultures. Folia Microbiol 31:154–163
Montague MD, Dawes EA (1974) The survival of Peptococcus prevotii in relation to the adenylate energy charge. J Gen Microbiol 80:291–299
Post E, Kleiner D, Oelze J (1983) Whole cell respiration and nitrogenase activities in Azotobacter vinelandii growing in oxygen controlled continuous culture. Arch Microbiol 134:68–72
Rubia T de la, Gonzalez-Lopez J, Moreno J, Martinez-Toledo MV, Ramos-Cormenzana A (1986a) Adenine nucleotide content and energy charge of Bacillus megaterium during batch growth in low-phosphate medium. FEMS Microbiol Letters 35:5–9
Rubia T de la, Gonzalez-Lopez J, Ballesteros F, Ramos-Cormenzana A (1986b) Growth of Bacillus megaterium in phosphate-limited medium. Folia Microbiol 31:98–105
Sadoff HI (1975) Encystment and germination in Azotobacter vinelandii. Bacteriol Revs 39:516–539
Tsai JC, Aladegbami SL, Vela GR (1979) Phosphate-limited culture of Azotobacter vinelandii. J Bacteriol 139:639–645
Yates MG (1970) Control of respiration and nitrogen fixation by oxygen and adenine nucleotides in N2-grown Azotobacter chroococcum. J Gen Microbiol 60:393–401
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de la Rubia, T., Gonzalez-Lopez, J., Moreno, J. et al. Adenine nucleotide contents and energy charge of Azotobacter vinelandii grown at low phosphate concentration. Arch. Microbiol. 147, 354–357 (1987). https://doi.org/10.1007/BF00406132
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DOI: https://doi.org/10.1007/BF00406132