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DNS/DNS-Homologiestudien innerhalb der Gattung Pediococcus

DNA-DNA homology studies on the genus Pediococcus

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Abstract

17 strains of Pediococcus, including new isolates and strains from various collections, were investigated by nucleic acid hybridisation. Most of the newly isolated strains, selected from about 500 strains according to physiological and biochemical characters, could be attributed to known species. The reassociation data demonstrate the separate status of P. pentosaceus, P. acidilactici, P. damnosus, P. parvulus and P. halophilus. The proposed neotype strain of P. acidilactici NCDO 1859, however, has been found to be in reality a genuine P. pentosaceus. P. dextrinicus, formerly described as P. cerevisiae var. dextrinicus showed a remarkable lack of relationship to any of the strains tested. Two isolates exhibiting a high phenotypic similarity to P. parvulus show little genetic relationship to this and especially to all other species.

Zusammenfassung

An 17 Stämmen der Gattung Pediococcus, die auf Grund charakteristischer physiologisch-biochemischer Merkmale aus einer Anzahl von etwa 500 Kulturen unterschiedlicher Herkunft ausgewählt wurden, werden mit Hilfe der DNS/DNS-Hybridisierung die verwandtschaftlichen Beziehungen untersucht. Dabei wird die Eigenständigeit der Arten P. pentosaceus, P. acidilactici, P. parvulus, P. damnosus und P. halophilus bestätigt. Der für P. acidilactici vorgeschlagene Neotyp-Stamm NCDO 1859 ist jedoch nach den vorliegenden Ergebnissen ein echter P. pentosaceus. Ein Stamm von P. dextrinicus, der früher bereits als P. cerevisiae var. dextrinicus beschrieben wurde, zeigt zu keiner untersuchten Art eine genetische Verwandtschaft auf der Basis der DNS/DNS-Homologie. Zwei aus Bier und Bierhefe isolierte Stämme, die hinsichtlich des Phänotyps P. parvulus nahestehen, zeigen weder zu dieser Art noch zu allen anderen bisher beschriebenen Arten hinsichtlich des Genotyps einen hohen Verwandtschaftsgrad.

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Abbreviations

CTAB:

N-cetyl-N,N,N-trimethyammoniumbromid

EDTA:

Äthylendiamintetraessigsäure

PPO:

2,5-Diphenyloxazol

POPOP:

1,4-Bis-(5-phenyl-2-oxazolyl)-benzol

SSC:

Saline-Trinatriumcitrat (0,15M NaCl, 0,015M Trinatriumcitrat, pH 7,0)

Tris:

Tris(hydroxymethyl)-aminomethan

T m :

mittlere Schmelztemperatur der DNS

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Authors and Affiliations

  1. Mikrobiologische Abteilung, Döhler GmbH & Co. KG, Riedstr. 9, D-6100, Darmstadt, Bundesrepublik Deutschland

    Werner Back

  2. Deutsche Sammlung von Mikroorganismen, Teilsammlung München, der Gesellschaft für Strahlen- und Umweltforschung mbH, Menzinger Str. 67, D-8000, München 19, Bundesrepublik Deutschland

    Erko Stackebrandt

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  1. Werner Back
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  2. Erko Stackebrandt
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Back, W., Stackebrandt, E. DNS/DNS-Homologiestudien innerhalb der Gattung Pediococcus . Arch. Microbiol. 118, 79–85 (1978). https://doi.org/10.1007/BF00406078

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