Summary
Xenotransplanted human breast-cancer tissue growing in the second to the ninth passage in nu/nu mice and normal tissue from nu/nu mice was cultivated in vitro. Thirty-two of fifty-two (62%) cultures grew successfully and have been analyzed. The cells were observed using phase-contrast microscopy and May-Grünwald-Giemsa staining. The following cell types could be distinguished: normal fibroblast-like cells (FC), normal epithelial-like cells (EP I), abnormal epithelial-like cells (EP II), and degenerated or dying cells (DC). After staining, the nuclear area of all cell types was measured. The results were: FC=280 μm2, EP I=320 μm2, and EP II=800 μm2. They were compared with the nuclear area of human breast-cancer cells obtained by aspiration biopsy.
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Volk, M., Scheuffele, J., Bastert, G. et al. In vitro cultivation of xenotransplanted human breast-cancer cells. J Cancer Res Clin Oncol 106, 179–186 (1983). https://doi.org/10.1007/BF00402605
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DOI: https://doi.org/10.1007/BF00402605