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Effect of mutations affecting lysyl-tRNALys on the regulation of lysine biosynthesis in Escherichia coli

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Summary

When studying mutants affecting lysyl-tRNA synthetase or tRNALys (hisT, hisW), a lack of correlation is clearly observed between the amount of lysyl-tRNA and the level of derepression of several lysine biosynthetic enzymes. This excludes the possible role of lysyl-tRNA as the specific corepressor of the lysine regulon. However, the level of derepression of DAP-decarboxylase, the last enzyme of the lysine pathway, is very low in the hisT mutant; this indicates that tRNALys is a secondary effector involved in the regulation of the synthesis of this enzyme.

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Abbreviations

DAP:

diaminopimelate

KRS:

lysyl-tRNA synthetase

L-lysine:

tRNA ligase (AMP) (EC6.1.16)

AK III:

lysinesensitive aspartokinase (EC 2.7.24)

ASA-dehydrogenase:

aspartic semialdehyde dehydrogenase (EC 1.2.1.10)

DHDP-reductase:

dihydrodipicolinic acid reductase

DAP-decarboxylase:

diaminopimelate decarboxylase (EC 4.1.1.20)

AK I:

threonine-sensitive aspartokinase

HDHI:

threonine-sensitive homoserine dehydrogenase

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Communicated by F. Gros

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Boy, E., Borne, F. & Patte, JC. Effect of mutations affecting lysyl-tRNALys on the regulation of lysine biosynthesis in Escherichia coli . Molec. Gen. Genet. 159, 33–38 (1978). https://doi.org/10.1007/BF00401745

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