Summary
The SCE frequency was studied in cultures of peripheral lymphocytes from three subjects before and after vaccination against measles. The immunological vaccination reactions were monitored by antibody titration and by measurement of DNA synthesis in peripheral lymphocytes. In two of the subjects, on the 14th day after vaccination, there was a marked decrease of the SCE frequency coinciding with common clinical vaccination reactions and an increase of DNA synthesis in the peripheral lymphocytes. The increase of antibody titers started on the 17th day. One month later, when the immunological reactions had subsided, the SCE frequency was increased by 25% over the prevaccination level. The third subject displayed a delayed vaccination response due to a simultaneous influenza infection. This subject showed a 50% increase in the SCE frequency on the 14th day as well as 6 weeks after vaccination. These results suggest that significant changes in the SCE frequency may be related to immunological vaccination reactions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ehrnst, A., Lambert, B., Fagraeus, A.: DNA synthesis in subpopulations of blood mononuclear leucocytes in human subjects after vaccination against yellow fever. Scand. J. Immunol. 8, 339–346 (1978)
Evans, H. J.: Some facts and fancies relating to chromosome structure in man. Adv. Hum. Genet. 8, 347–438 (1977)
Harnden, D. G.: Viruses, chromosomes, and tumours: The interaction between viruses and chromosomes. In: Chromosomes and cancer, J. German (ed.), pp. 151–190. New York: Wiley 1974
Kato, H., Sandberg, A. A.: Effects of herpes simplex virus on sister chromatid exchange and chromosome abnormalities in human diploid fibroblasts. Exp. Cell Res. 109, 423–427 (1977)
Knuutila, S., Mäki-Pakkanen, J., Kähkönen, M., Hokkanen, E.: An increased frequency of chromosomal changes and SCEs in cultured blood lymphocytes of 12 subjects vaccinated against smallpox. Hum. Genet. 41, 89–96 (1978)
Lambert, B., Hansson, K., Lindsten, J., Sten, M., Werelius, B.: Bromodeoxyuridine-induced sister chromatid exchanges in human lymphocytes. Hereditas 83, 163–174 (1976)
Lambert, B., Lindblad, A., Nordenskjöld, M., Werelius, B.: Increased frequency of sister chromatid exchanges in cigarette smokers. Hereditas 88, 147–149 (1978a)
Lambert, B., Ringborg, U., Harper, E., Lindblad, A.: Sister chromatid exchanges in lymphocyte cultures of patients receiving chemotherapy for malignant disorders. Cancer Treat. Rep. 62, 1413–1419 (1978b)
Lambert, B., Ringborg, U., Lindblad, A.: Prolonged increase of sister chromatid exchanges in lymphocytes of melanoma patients after CCNU treatment. Mutat. Res. 59, 295–300 (1979)
Nevstad, N. P.: Sister chromatid exchanges and chromosomal aberrations induced in human lymphocytes by the cytostatic drug adriamycin in vivo and in vitro. Mutat. Res. 57, 253–258 (1978)
Norrby, E., Gollmar, Y.: Identification of measles virus-specific chemolysis-inhibiting antibodies separate from hemagglutination-inhibiting antibodies. Infect. Immun. 11, 231–239 (1975)
Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature 251, 156–158 (1974)
Raposa, T.: Sister chromatid exchange studies for monitoring DNA damage and repair capacity after cytostatic in vitro and in lymphocytes of leukaemic patients under cytostatic therapy. Mutat. Res. 57, 253–258 (1978)
Wolff, S.: Sister chromatid exchange. Ann. Rev. Genet. 11, 183–201 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lambert, B., Ehrnst, A., Hansson, K. et al. Sister chromatid exchange in peripheral lymphocytes of subjects vaccinated against measles. Hum Genet 50, 291–296 (1979). https://doi.org/10.1007/BF00399395
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00399395