Abstract
Calcium transport by purified endomembrane vesicles from roots of Pisum sativum L. was studied. Two types of ATP-dependent transport were demonstrated, protonophore-sensitive calcium/proton antiport and protonophore-insensitive transport. The former was predominantly located in the tonoplast, while the latter was associated with the plasma membrane and Golgi apparatus. Calcium uptake by membranes of low buoyant density (tonoplast) was inhibited by nitrate and was not sensitive to sodium orthovanadate while that by membranes of high buoyant density (plasma membrane, Golgi apparatus) was inhibited by sodium orthovanadate and insensitive to nitrate. Endoplasmic reticulum also took up calcium in the presence of ATP; however, because of its imperfect separation from the tonoplast, the nature of the calcium-transport system present could not be fully characterised. None of the calcium transporters were stimulated by calmodulin or inhibited by calmodulin inhibitors.
Similar content being viewed by others
Abbreviations
- FCCP:
-
carbonyl cyanide-p-trifluoromethoxyphenyl hydrozone
References
Balke, N.E., Hodges, T.K. (1979) Inhibition of adenosine triphosphatase activity of the plasma membrane fraction of oat roots by diethyl-stilbestrol. Plant Physiol. 63, 48–52
Blumwald, E., Poole, R.J. (1986) Kinetics of Ca2+/H+ antiport in isolated tonoplast vesicles from storage tissue of Beta vulgaris L. Plant Physiol. 80, 727–731
Bowman, B.J., Slayman, C.W. (1979) The effect of vanadate on the plasma membrane ATPase of Neurospora crassa. J. Biol. Chem. 254, 2928–2934
Buckhout, T.J. (1983) ATP-dependent CA2+ transport in endoplasmic reticulum isolated from roots of Lepidium sativum L. Planta 159, 84–90
Bush, D.R., Sze, H. (1986) Calcium transport in tonoplast and endoplasmic reticulum vesicles isolated from cultured carrot cells. Plant Physiol. 80, 549–555
Butcher, R.D., Evans, D.E. (1987) Calcium transport by pea root membranes. I. Purification of membranes and characteristics of uptake. Planta 172, 265–272
Clarkson, D.T. (1986) Future development of calcium studies; evidence in the support of concepts. In: Molecular and cellular aspects of calcium in plant development, pp. 167–174, Trewavas, A.J., ed. Plenum, New York London
Dieter, P., Marmé, D. (1980a) Ca2+ transport in mitochondrial and microsomal fractions from higher plants. Planta 150, 1–8
Dieter, P., Marmé, D. (1980b) Calmodulin activation of plant microsomal calcium uptake. Proc. Natl. Acad. Sci. USA 77, 7311–7314
Dieter, P., Marmé, D. (1981a) Far-red light irradiation of intact corn seedlings affects mitochondrial and calmodulin dependent micrsomal transport. Biochem. Biophys. Res. Commun. 101, 749–755
Dieter, P., Marmé, D. (1981b) A calmodulin-dependent, microsomal ATPase from corn (Zea mays L.) FEBS Lett 125, 245–248
Gabathuler, R., Cleland, R.E. (1985) Auxin regulation of a proton translocating ATPase in pea root plasma membrane vesicles. Plant Physiol 79, 1080–1085
Hager, A. Hermsdorf, P. (1981) A H+/Ca2+ antiporter in membranes of microsomal vesicles from maize coleoptiles, a secondary energised Ca pump. Z. Naturforsch. 36c, 1009–1012
Harmon, A.C., Jarrett, H.W., Cormier, M.J. (1984) An enzymatic assay for calmodulin based on plant NADkinase activity. Anal. Biochem. 141, 168–178
Moloney, M.M., Henri, H., Pilet, P.E. (1983) Regulation of in vitro H+ transport in sealed pea root vesicles. Plant Physiol. 72, 5140
Rasi-Caldogno, F., DeMichelis, M.E., Pugliarello, M.C. (1982) Active transport of Ca in membrane vesicles from pea. Evidence for a H+/Ca2+ antiport. Biochim. Biophys. Acta 693, 287–295
Schumaker, K.S., Sze, H. (1985) A Ca2+/H+ antiport system driven by the proton electrochemical gradient of a tonoplast H+ ATPase from oat roots. Plant Physiol. 79, 1111–1117
Sze, H. (1985) H+ translocating ATPase: advances using membrane vesicles. Annu. Rev. Plant Physiol. 36, 175–396
Zocchi, G., Hanson, J.B. (1983) Calcium transport and ATPase activity in a microsomal vesicle fraction from corn roots. Plant Cell Environ. 6, 203–209
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Butcher, R.D., Evans, D.E. Calcium transport by pea root membranes. Planta 172, 273–279 (1987). https://doi.org/10.1007/BF00394597
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00394597