Abstract
Four S-adenosyl-l-methionine:anthocyanin-3′,5′-O-methyltransferases in flowers of Petunia hybrida were separated using the chromatofocusing technique. Each methyltransferase is controlled by one of the methylation genes Mt1, Mt2, Mf1 or Mf2. Molecular weight, pH-activity optimum, isoelectric point, several kinetic properties and the behaviour in the presence of Mg2+, ethylenediaminetetraacetic acid and S-adenosyl-l-homocysteine of each of the four enzymes were determined. The methylation in vitro of delphinidin 3-(p-coumaroyl)-rutinosido-5-glucoside reflected the accumulation patterns of methylated anthocyanins in vivo and established the regulatory role of methyltransferases in vivo.
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Abbreviations
- EDTA:
-
ethylenediaminetetraacetic acid
- OMT:
-
O-methyltransferase
- 3RGac5G:
-
3-(p-coumaroyl)-rutinosido-5-glucoside
- SAH:
-
S-adenosyl-l-homocysteine
- SAM:
-
S-adenosyl-l-methionine
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Jonsson, L.M.V., Aarsman, M.E.G., Poulton, J.E. et al. Properties and genetic control of four methyltransferases involved in methylation of anthocyanins in flowers of Petunia hybrida . Planta 160, 174–179 (1984). https://doi.org/10.1007/BF00392867
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DOI: https://doi.org/10.1007/BF00392867