Abstract
Cell homogenates from Catharanthus roseus (L.) G. Don. grown S-autotrophically on sulfate in the dark are capable of reducing adenylysulfate (APS) to cysteine. This reduction required a particulate protein fraction from the cell extract and reduced ferredoxin as the electron donor. The protein fraction (MW 700,000±50,000) was found to contain Fd:NADP+ reductase, glutathione reductase and an unspecific dithiol reductase, and APS-sulfotransferase and thiosulfonate reductase activity. Resolution into these individual enzyme activities led to a non-restorable loss of the APS reducing activity. It was observed that a slow gradual decay of the APS reducing activity was accompanied by a likewise slow generation of a ferredoxin-dependent sulfite reductase.
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Abbreviations
- APS:
-
Adenosine 5′-phosphosulfate
- APS-kinase:
-
E.C.2.7.1.25
- ATP-sulfurylase:
-
E.C.2.7.7.4
- Fd:
-
ferredoxin
- Fd-NADP+-reductase:
-
E.C.1.6.7.1.
- Glutathione reductase:
-
E.C.1.6.4.2.
- G6P:
-
Glucose 6-phosphate
- G6PDH:
-
glucose 6-phosphate dehydrogenase, E.C.1.1.49
- GSSG:
-
oxidized glutathione
- GSSO3H:
-
S-sulfoglutathione
- MVH:
-
reduced methylviologen
- OASS:
-
O-acetylserine sulfhydrylase-E.C. 4.2.99.8
- Sulfite reductase:
-
E.C.1.8.1.2
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Urlaub, H., Jankowski, G. Sulfate reduction in Catharanthus roseus (L.). Planta 155, 154–161 (1982). https://doi.org/10.1007/BF00392546
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DOI: https://doi.org/10.1007/BF00392546