Abstract
The biochemical lesion in a light-sensitive, acetate-requiring Chlamydomonas mutant was identified. This strain, designated rpk, exhibited photosynthetic rates less than 3% of the wild-type. Analysis of photosynthetic products by high-performance liquid chromatography demonstrated an accumulation of 14C label in pentose and hexose monophosphates. After 1 min of photosynthesis in 14CO2 these intermediates comprised 27.5% of the label in the mutant compared with 8% in the wild-type. The mutant pheno-type was caused by a 20-fold reduction in ribulose-5-phosphate (Ru5P)-kinase (EC 2.7.1.19) activity. The mutant exhibited wild-type levels of ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39), fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) and transketolase (EC 2.2.1.1) indicating that the mutation specifically affected Ru5P kinase. In a cross of the mutant with the wild-type, tetrad progeny segregated in a Mendelian fashion (1:1) and light-sensitivity cosegregated with reduced Ru5P-kinase activity and an acetate requirement for growth. Almost normal levels of Ru5P-kinase protein were detected in the mutant by probing nitrocellulose replicas of sodium dodecylsulfate-polyacrylamide gels with anti-Ru5P-kinase antibody. The subunit size of the mutant enzyme, 42 kDa, was identical to that of the wild-type. Isoelectric focusing of the native protein determined that the mutant protein was altered, exhibiting a more acidic isoelectric point than the wild-type protein. Thus, the molecular basis for the lesion affecting Ru5P-kinase activity in mutant rpk is a charge alteration which results in a partially impaired enzyme.
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Abbreviations
- Chl:
-
chlorophyll
- Da:
-
dalton
- FCCP:
-
carbonylcyanide-p-trifluorophenylhydrazone
- RuBP:
-
ribulose-1,5-bisphosphate
- Ru5P:
-
ribulose-5-phosphate
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Salvucci, M.E., Ogren, W.L. A Chlamydomonas reinhardii mutant with catalytically and structurally altered ribulose-5-phosphate kinase. Planta 165, 340–347 (1985). https://doi.org/10.1007/BF00392230
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DOI: https://doi.org/10.1007/BF00392230