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Association of macrophages detected with monoclonal antibody 25 F 9 with progression and pathobiological classification of gastric carcinoma

  • Original Papers
  • Clinical Oncology or Epidemiology
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Summary

Using monoclonal antibody 25 F 9, which reacts with a determinant of mature macrophages, the inflammatory infiltrate of 66 gastric carcinomas was evaluated using a counting grid. The ratio tumor cells/macrophages was determined for every tumor. For a threshold value of 5, carcinomas with a better prognosis, such as the intestinal type according to Lauré, the expanding type according to Ming and the differentiated carcinomas according to the WHO had a significantly smaller relative content of 25 F 9-positive macrophages (a minimum of P<0.05) than the diffuse type, infiltrative type, and undifferentiated carcinomas. Furthermore, the relative macrophage content tended to increase with the stage of carcinoma spread (P<0.1). The results suggested that 25 F 9-positive macrophages in gastric carcinoma are of greater significance in tumor spread than in any defensive reaction against the tumor.

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References

  1. Allen C, Hogg N (1985) Monocytes and other infiltrating cells in human colorectal tumours identified by monoclonal antibodies. Immunology 55:289–299

    Google Scholar 

  2. Biondi A, Rossing TH, Bennett J, Todd RF III (1984) Surface membrane heterogeneity among human mononuclear phagocytes. J Immunol 132:1237–1243

    Google Scholar 

  3. Birbeck MSC, Carter RL (1972) Observations on the ultrastructure of two hamster lymphomas with particular reference to infiltrating macrophages. Int J Cancer 9:249–257

    Google Scholar 

  4. Black MM, Freeman C, Mork T, Harvel S, Cutler SJ (1971) Prognostic significance of microscopic structure of gastric carcinomas and their regional lymph nodes. Cancer 27:703–711

    Google Scholar 

  5. Bröcker E-B, Kolde G, Steinhausen D, Peters A, Macher E (1984) The pattern of the mononuclear infiltrate as a prognostic parameter in flat superficial spreading melanomas. J Cancer Res Clin Oncol 107:48–52

    Google Scholar 

  6. Bröcker E-B, Zwadlo G, Suter L, Brune M, Sorg C (1987) Infiltration of primary and metastatic melanomas with macrophages of the 25 F 9-positive phenotype. Cancer Immunol Immunother 25: (in press)

  7. Brooks DA, Zola H, McNamara PJ, Bradley J, Bradstock KF, Hancock WW, Atkins RC (1983) Membrane antigens of human cells of the monocyte/macrophage lineage studied with monoclonal antibodies. Pathology 15:45–52

    Google Scholar 

  8. Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ (1983) Human mononuclear phagocyte differentiation antigens. 1. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol 130:145–152

    Google Scholar 

  9. Eccles SA, Alexander P (1974) Macrophage content of tumors in relation to metastatic spread and host immune reaction. Nature 250:667–669

    Google Scholar 

  10. Evans R, Duffy T, Cullen RT (1984) Tumor-associated macrophages stimulate the proliferation of murine tumor cells surviving treatment with the oncolytic cyclophosphamide analogue asta Z-7557: in vivo implications. Int J Cancer 34:883–890

    Google Scholar 

  11. Gauci CL (1976) The significance of the macrophage content of human tumours. In: Mathé G, Florentin I, Simmler MC (eds) Lymphocytes, macrophages, and cancer Springer Berlin Heidelberg New York, pp 122–130

    Google Scholar 

  12. Gauci CL, Alexander P (1975) The macrophage content of some tumours. Cancer Lett 1:29–32

    Google Scholar 

  13. Grüter Th, Heidl G, Grundmann E, Zwadlo G, Sorg C (1985) Immunhistochemische Untersuchungen des Makrophageninfiltrates in Magenkarzinomen. Verh Dtsch Ges Pathol 69:460

    Google Scholar 

  14. Heild G, Davaris P, Düchting, S, Jagoda MS, Bierhoff E, Krieg V, Grundmann E (1986) Zur Nachweisbarkeit des karzinoembryonalen Antigens (CEA) an Gefrier-und Paraffinschnitten von Magenkarzinomen. Verh Dtsch Ges Pathol 70:225–230

    Google Scholar 

  15. Hibbs Jb, Chapman H Jr, Weinberg JB (1978) The macrophage as an antineoplastic surveillance cell: biological perspecitves. J Reticuloendothel Soc 24:549–570

    Google Scholar 

  16. Hogg N, MacDonald M, Slusarenko M, Beverly PCL (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology 53:753–767

    Google Scholar 

  17. Immich H (1974) Medizinische Statistik. Eine Einführungsvorlesung. Schattauer, Stuttgart New York

    Google Scholar 

  18. Inberg M, Laurén V, Vuori J, Viikari SJ (1973) Prognosis in intestinal-type and diffuse gastric carcinoma with special reference to the effort of the stromal reaction. Acta Chir Scand 139:273–278

    Google Scholar 

  19. Inokuchi K, Inutsuka S, Furusawa M (1967) Stroma reaction around tumor and metastasis and prognosis after curative gastrectomy for carcinoma of the stomach. Cancer 120:1924–1929

    Google Scholar 

  20. Keller R (1973) Cytostatic elimination of syngeneic rat tumor cells in vitro by nonspecifically activated macrophages. J Exp Med 138:625–644

    Google Scholar 

  21. Kopper L, Lapis K (1985) What's new in macrophage tumor cell interaction? Pathol Res Pract 179:652–655

    Google Scholar 

  22. Knowles DM, Tolidjian IB, Marboe C, D'Agati V, Grimes M, Chess L (1984) Monoclonal anti-human monocyte antibodies OKM 1 and OKM 5 possess distinctive tissue distributions inclunding differential reactivity with vascular endothelium. J Immunol 132:2170–2173

    Google Scholar 

  23. Lapis K, Kopper L, Timar J, van Hanh T, Hegedüs C (1981) Effect of macrophages on the growth of syn-and xenogeneic tumors. Arch Geschwulstforsch 51:475–479

    Google Scholar 

  24. Lasser A (1983) The mononuclear phagocyte system: a review. Hum Pathol 14:108–126

    Google Scholar 

  25. Lauder I, Waherne J, Stewart J, Sainsbury R (1977) Macrophage infiltration of breast tumors: a prospective study. J Clin Pathol 30:563–568

    Google Scholar 

  26. Laurén P (1965) The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma. An attempt at a histo-clinical classification. Acta Pathol Microbiol Scand 64:31–49

    Google Scholar 

  27. Mahoney KH, Fulton AM, Heppner GH (1983) Tumor-associated macrophages of mouse mammary tumors. II. Differential distribution of macrophages from metastatic and nonmetastatic tumors. J Immunol 131:2079–2085

    Google Scholar 

  28. Mantovani A, Giavazzi R, Polentaruti N, Spreafico F, Garattini S (1980) Divergent effects of macrophage toxins on growth of primary tumors and lung metastases in mice. Int J Cancer 25:617–620

    Google Scholar 

  29. Maruyama S, Naito T, Kakita H, Kishimoto S, Yamamura Y, Kishimoto T (1983) Preparation of a monoclonal antibody agains human monocyte lineage. J Clin Immunol 3:57–64

    Google Scholar 

  30. Mehrotra ML, Gupta IM, Khanna S, Vaidya MP (1978) Host response and tumor biological behavior in the two histological types of gastric carcinoma. Histopathology 2:373–382

    Google Scholar 

  31. Ming S-C (1977) Gastric carcinoma. A pathobiological classification. Cancer 39:2475–2485

    Google Scholar 

  32. Monafo WW, Krause GL, Medina JG (1962) Carcinoma of the stomach: morphological characteristics affecting survival. Arch Surg 85:754–763

    Google Scholar 

  33. Nacopoulou L, Azaris P, Papacharalampous N, Davaris P (1981) Prognostic significance of histological host response in cancer of the large bowel. Cancer 47:930–936

    Google Scholar 

  34. Nash JRG (1982) Macrophages in human tumours: an immunohistochemical study. J. Pathol 136:73–83

    Google Scholar 

  35. Nathan CF, Murray HW, Cohn ZA (1980) Current concepts: the macrophage as an effector cell. New Engl J Med 303:622–626

    Google Scholar 

  36. Neumann C, Sorg C (1983) Regulation of plasminogen activator secretion, interferon induction and proliferation in murine macrophages. Eur J Immunol 13:143–147

    Google Scholar 

  37. North RJ (1978) Opinions: the concept of the activated macrophage. J Immunol 121:806–816

    Google Scholar 

  38. Okada M, Kojima S, Murakami M, Fuchigami T, Yao T, Omae T, Iwashita A (1983) Human gastric carcinoma: prognosis in relation of macroscopic and microscopic features of the primary tumor. J Natl Cancer Inst 71:275–279

    Google Scholar 

  39. Oota K, Sobin LH (1977) Histological typing of gastric and oesophageal tumors. Int Histol Class of Tumours; 18. World Health Organization, Geneva

    Google Scholar 

  40. Pagnini CA, Rugge M (1985) Advanced gastric cancer and prognosis. Virchow's Arch (Pathol Anat) 406:213–221

    Google Scholar 

  41. Plytycz B, Pedersen A, Bogwald J, Seljelid R (1986) In vitro interactions of murine peritoneal macrophages and sarcoma cells. I. Promotion of tumor cell proliferation by macrophages. Virchows Arch (Cell Pathol) 50:285–292

    Google Scholar 

  42. Polverini PJ, Cotran RS, Gimbrone MA, Unanue ER (1977) Activated macrophages induce vascular proliferation. Nature 269:804–806

    Google Scholar 

  43. Radzun HJ, Parwaresch MR (1983) Differential immunohistochemical resolution of the human mononuclear phagocyte system. Cell Immunol 82:174–183

    Google Scholar 

  44. Raff HV, Picker LJ, Stobo JD (1980) Macrophage heterogeneity in man. A subpopulation of HLA-DR-bearing macrophages required for antigen-induced T-cell activation also contains stimulators for autologous-reactive T-cells. J Exp Med 152:581–593

    Google Scholar 

  45. Ribeiro MM, Sarmento JA, Simoes MA, Bastos J (1981) Prognostic significance of Laurén and Ming classifications and other pathologic parameters in gastric carcinoma. Cancer 47:780–784

    Google Scholar 

  46. Stemmermann GN, Brown C (1974) A survival study of intestinal and diffuse types of gastric carcinoma. Cancer 33:1190–1195

    Google Scholar 

  47. Stenzinger W, Brüggen J, Macher E, Sorg C (1983) Tumor angiogenic activity (TAA) production in vitro and growth in the nude mouse by human malignant melanoma. Eur J Cancer Clin Oncol 19 (5):649–656

    Google Scholar 

  48. Stosiek P, Varga A, Kasper M (1983) Untersuchungen zur immunologischen Bedeutung der entzündlichen Stromareaktion bösartiger Tumoren. Klin Wochenschr 61:157–162

    Google Scholar 

  49. Stosiek P, Kasper M, Varga A (1983) Prognoserelevante Befunde beim Magenkarzinom (Varianz-und diskriminanzanalytische Untersuchungen mit klinischen, morphologischen und immunologischen Merkmalen). Arch Geschwulstforsch 53:61–67

    Google Scholar 

  50. Stutman O (1975) Immunosuppression and malignancy. Adv Cancer Res 22:261–422

    Google Scholar 

  51. Svennevig J-L, Lövik M, Svaar H (1979) Isolation and characterization of lymphocytes and macrophages from solid, malignant human tumours. Int J Cancer 23:626–631

    Google Scholar 

  52. Svennevig J-L, Lunde OC, Holter J, Bjorgsvik D (1984) Lymphoid infiltration and prognosis in colorectal carcinoma. Br J Cancer 49:375–377

    Google Scholar 

  53. Talle MA, Rao PE, Westberg E, Allegar N, Makowski M, Mittler RS, Goldstein G Patterns of antigenic expression on human monocytes as defined by monoclonal antibodies. Cell Immunol 78:83–99

  54. Ting CC, Rodriguez D (1980) Switching on the macrophage-mediated suppressor mechanism by tumor cells to evade host immune surveillance. Proc Natl Acad Sci 77:4265–4269

    Google Scholar 

  55. Todd RF III, Nadler LM, Schlossman SF (1983) Antigens on human monocytes identified by monoclonal antibodies. J Immunol 126:1435–1442

    Google Scholar 

  56. Ugolini V, Nunez G, Smith RG, Stastny P, Capra JD (1980) Initial characterization of monoclonal antibodies against human monocytes. Proc Natl Acad Sci USA (Immunol) 77:6764–6768

    Google Scholar 

  57. UICC (1985) TNM Atlas, Illustrierter Leitfaden zur TNM/pTNM-Klassifikation maligner Tumoren. Hrsg. B. Spiessl, P. Hermanek, O. Scheibe, G. Wagner. Springer, Berlin Heidelberg NY Tokyo

    Google Scholar 

  58. Vollmer E, Grundmann E, Nienhaus H, Mellin W, Zwadlo G (1985) Macrophages and proliferation in mammary carcinoma and mastopathia. An immunohistochemical study. Verh Dtsch Ges Pathol 69:135–142

    Google Scholar 

  59. Watanabe H, Enjoji M, Imal T (1976) Gastric carcinoma with lymphoid stroma. Cancer 38:232–243

    Google Scholar 

  60. Weibel ER (1967) Structure in space and its appearance in sections. In: Elias H (ed) Stereology. Springer, New York

    Google Scholar 

  61. Wood GW, Collahon KA (1979) Detection and quantification of macrophage infiltration into primary human tumors with the use of cell-surface markers. J Natl Cancer Inst 59:1081–1087

    Google Scholar 

  62. Zwaldo G, Bröcker E-B, von Bassewitz D-B, Feige U, Sorg C (1985) A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol 134:1487–1497

    Google Scholar 

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Authors and Affiliations

  1. Gerhard-Domagk-Institute of Pathology, Domagkstraße 17, D-4400, Münster, Federal Republic of Germany

    G. Heidl, P. Davaris, M. S. Jagoda, S. Düchting, E. Bierhoff, T. Grüter & V. Krieg

  2. Department of Experimental Dermatology, Dermatologic Hospital, Westfälische Wilhelms-Universität, D-4400, Münster, Federal Republic of Germany

    G. Zwadlo & C. Sorg

Authors
  1. G. Heidl
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  2. P. Davaris
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  4. M. S. Jagoda
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  5. S. Düchting
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  6. E. Bierhoff
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  7. T. Grüter
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  8. V. Krieg
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  9. C. Sorg
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Dedicated to Professor Dr.E. Grundmann (Münster, FRG) on the occasion of his 65th birthday

Supported by the Deutsche Forschungsgemeinschaft (AZ Gr 711/2-1)

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Heidl, G., Davaris, P., Zwadlo, G. et al. Association of macrophages detected with monoclonal antibody 25 F 9 with progression and pathobiological classification of gastric carcinoma. J Cancer Res Clin Oncol 113, 567–572 (1987). https://doi.org/10.1007/BF00390867

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  • DOI: https://doi.org/10.1007/BF00390867

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