Skip to main content
SpringerLink
Log in

Untersuchung der Lichtabhängigkeit der Aufnahme von Rubidium, Zink, Kobalt, Blei und Cer durch Chlorella nach einer Flußmethode

Investigation of the light dependence of the uptake of rubidium, zinc, cobalt, lead and cerium by Chlorella by a flow method

  • Published:
Planta Aims and scope Submit manuscript

Summary

A flow method for the measurement of the uptake of labelled substances by plant cells has been developed. Accumulation in the medium of substances released by the plants is thereby avoided, and a more precise determination of the rate of uptake—also of transients—than through the usual, static methods is possible. The time function of the stimulation of the uptake of Rb by Chlorella fusca by light at 30° has been analyzed, and the component believed to represent active transport separated from other components that have shorter time constants; at 3° the active component is much reduced.

In the cases of the heavy metal ions Zn, Co, Pb and Ce, even after presaturation with the ions in the dark for the suppression of the short-term uptake the light-independent long-term uptake is far stronger than with Rb. Moreover, for Zn and Co the uptake is greatly stimulated by light at 30°, but little if at all at 5°. No stimulation was found under any conditions with Ce. With Pb, the light effect was hardly reproducible, and in any case extremely small. The energy-dependent transport of Zn and Co is probably active.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Barber, J.: The influx of potassium into Chlorella pyrenoidosa. Biochim. biophys. Acta (Amst.) 163, 141–149 (1968).

    Google Scholar 

  • Broda, E.: The uptake of some heavy trace elements by Chlorella. In: Stofftransport und Stoffverteilung in Zellen höherer Pflanzen (Hrsg. K. Mothes, E. Müller, A. Nelles, D. Neumann), S. 109–116. Berlin: Akademie-Verlag 1968.

    Google Scholar 

  • Brown, F., Cuthbertson, W. F. J., Fogg, G. E.: Vitamin B12 activity of Chlorella vulgaris Beij. and Anabaena cylindrica Lemm. Nature (Lond.) 177, 188 (1956).

    Google Scholar 

  • Findenegg, G. R.: Aktive und passive Aufnahme von Spurenelementen durch Chlorella. Diss. Wien 1968.

  • — Broda, E.: Stoffwechselabhängige Aufnahme von Zink durch Chlorella. Naturwissenschaften 53, 358–359 (1966).

    PubMed  Google Scholar 

  • —, Springer-Lederer, H.: Apparat zur laufenden Gewinnung von Chlorella-Algen für chemische Untersuchungen. Allg. prakt. Chem. 18, 279–280 (1967).

    Google Scholar 

  • Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., Singh, R. M.: Hydrogen ion buffer for biological research. Biochemistry (Wash.) 5, 467–477 (1966).

    Google Scholar 

  • Lüttge, U.: Aktiver Transport. In: M. Alfert, H. Bauer, C. V. Harding, W. Sandritter, P. Sitte (Hrsg.), Protoplasmatologia, Bd. VIII/7b, S. 40ff. Wein: Springer 1969.

    Google Scholar 

  • Matzku, S., Broda, E.: Die Zinkaufnahme in das Innere von Chlorella. Planta (Berl.) 92, 29–40 (1970).

    Google Scholar 

  • Müller, E., Paschinger, H.: Action of inhibitors of protein synthesis on active transport. Biochem. Physiol. Pflanzen (Jena) 161, 476–480 (1970).

    Google Scholar 

  • Nightingale, E. R.: Phenomenological theory of ion solvation. Effective radii of hydrated ions. J. Physic. Chem. 63, 1381–1387 (1959).

    Google Scholar 

  • Packer, L., Murakami, S., Mehard, C. W.: Ion transport in chloroplasts and plant mitochondria. Ann. Rev. Plant Physiol. 21, 271–304 (1970).

    Article  Google Scholar 

  • Paschinger, H., Broda, E.: Hemmung der aktiven Bromidaufnahme durch Pflanzenzellen durch Glucose. Mh. Chem. 98, 905–910 (1967).

    Google Scholar 

  • Ruppel, H. C.: Untersuchungen über die Zusammensetzung von Chlorella bei Synchronisation im Licht-Dunkel-Wechsel. Flora (Jena) 152, 113–138 (1962).

    Google Scholar 

  • Schuster, I., Broda, E.: Die Bindung von Zink durch Zellwände von Chlorella. Mh. Chem. 101, 285–295 (1970).

    Google Scholar 

  • Springer-Lederer, H., Rosenfeld, D. L.: Energy sources for the absorption of rubidium by Chlorella, Physiol. Plantarum (Cph.) 21, 435–444 (1968).

    Google Scholar 

  • Wiessner, W.: Light effects on ion fluxes in microalgae. In: P. Halldal (ed.), Photobiology of microorganisms. Chichester: J. Wiley & Sons 1970.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Radiochemische Abteilung, Institut für Physikalische Chemie, Universität Wien, Wien, Österreich

    G. R. Findenegg, H. Paschinger & E. Broda

  2. Institut für Physikalische Chemie, Währingerstr. 42, A-1090, Wien, Österreich

    G. R. Findenegg, H. Paschinger & E. Broda

Authors
  1. G. R. Findenegg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Paschinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Broda
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Herrn Prof. Dr. K. Mothes zum 70. Geburtstag in Verehrung gewidment.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Findenegg, G.R., Paschinger, H. & Broda, E. Untersuchung der Lichtabhängigkeit der Aufnahme von Rubidium, Zink, Kobalt, Blei und Cer durch Chlorella nach einer Flußmethode. Planta 99, 163–173 (1971). https://doi.org/10.1007/BF00388248

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00388248

Search

Navigation