Abstract
Polyadenylic acid [poly (A)] is detected, characterized and quantitated in dry radish embryo axis RNA using a 3H poly (U) probe. The amount of poly (A) gradually decreases after the onset of soaking, and, after a few hours, recovers to the initial level. This variation is shown to result from the addition of two opposed phenomena: the decay of stored poly (A) and the accumulation of newly synthesized poly (A). Stored poly (A), as well as the “in vivo” protein synthesis coded for by preformed mRNA, decreases during early germination with a half-life of two hours. As a whole, these results demonstrate that at least a fraction of the stored mRNA is translated as soon as the seed is soaked and that its role is rapidly taken over by newly-made mRNA.
Similar content being viewed by others
Abbreviations
- Poly (A) (+) RNA:
-
polyadenylated RNA
- Poly (A):
-
polyadenylic acid
- Poly (U):
-
polyuridylic acid
References
Bishop, J.O., Rosbash, M., Evans, D.: Polynucleotide sequences in eucaryotic DNa and RNA that form ribonuclease-resistant complexes with polyuridylic acid. J. Mol. Biol. 85, 75–86 (1974)
Delseny, M., Aspart, L., Balat, H., Guitton, Y.: Détection de séquences polyribo-adényliques dans les ARN de Radis. C.R. Acad. Sci. (Paris) 278, série D, 1225–1228 (1974)
Delseny, M., Peralta, M.T., Guitton, Y.: Effects fo cordycepin on RNA metabolism in germinating seedlings. Biochem. Biophys. Res. Commun. 64, 1278–1285 (1975)
Delseny, M., Got, A., Guitton, Y.: Phosphorylation de la thymidine au cours de la germination du Radis par une nucléoside AMP-phosphotransférase synthétisée de novo: mise en évidence d'un décalage dans le temps entre la transcription du géne et son expression. Physiol. Vég. 14, 159–178 (1976a)
Delseny, M., Aspart, L., Got, A., Guitton, Y.: Messenger RNA metabolism in germinating radish seedlings. In: Colloques Internationaux CNRS no 261. Acides nucléiques et synthèse des protéines chez les végétaux, pp. 521–525. Weil, J.H., Bogorad, L., eds. Paris: Editions du CNRS 1976b
Delseny, M., Aspart, L., Guitton, Y.: Effect of the protein synthesis inhibitor cycloheximide on RNA synthesis in Radish seedlings. Biochimie 59, 51–57 (1977)
Dure, L.S. III, Harris, B.: Regulation of translation of stored mRNA of dicot seeds. In: Nato Symposium on nucleic acids and protein synthesis in plants. Weil, J.H., Bogorad, L., eds. In press. New-York: Plenum publishing Corp. 1976
Glisin, V., Crvenjakov, R., Byus, C.: Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13, 2633–2637 (1974)
Gordon, M.E., Payne, P.I.: In vitro translation of the long-lived messenger ribonucleic acid of dry seeds. Planta 130, 269–273 (1976)
Hammet, J.R., Katterman, F.R.: Storage and metabolism of poly (adenylic acid)- mRNA in germinating cotton seeds. Biochemistry 14, 4375–4379 (1975)
Lindberg, U., Persson, T.: Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to sepharose. Europ. J. Biochem. 31, 246–254 (1972)
Marcus, A., Spiegel, S., Brooker, J.D.: Preformed mRNA and the programming of early embryo development. In: Control Mechanisms in Development, pp. 1–19. Meints, R.H., Davies, E., eds. New York: Plenum publishing Corp. 1975
Payne, P.I.: The long-lived messeneer ribonucleic acid of floweringplant seeds. Biol. Rev. 51, 329–363 (1976)
Rosbash, M., Ford, P.J.: Polyadenylic acid-containing RNA in Xenopus laevis oocytes. J. Mol. Biol. 85, 87–101 (1974)
Spiegel, S., Marcus, A.: Polyribosome formation in early wheat embryo germination independent of either transcription or polyadenylation. Nature 256, 228–230 (1975)
Spiegel, S., Obendorf, R.L., Marcus, A.: Transcription of ribosomal and messenger RNAs in early wheat embryo germination. Plant Physiol. 56, 502–507 (1975)
Walbot, V., Harris, B., Dure, L.S. III: The regulation of enzyme synthesis in the embryogenesis and germination of cotton. In: Developmental Biology of reproduction, pp. 165–187. Markert, C., ed. New York: Academic Press 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Delseny, M., Aspart, L. & Guitton, Y. Disappearance of stored polyadenylic acid and mRNA during early germination of radish (Raphanus sativus L.) embryo axes. Planta 135, 125–128 (1977). https://doi.org/10.1007/BF00387160
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387160