Skip to main content
SpringerLink
Log in

Die Wirkung von Dinactin auf Volumenänderungen ganzer Chloroplasten im Licht und im Dunkeln

The action of dinaction on volume changes of whole chloroplasts in light and dark

  • Published:
Planta Aims and scope Submit manuscript

Summary

  1. 1.

    Whole chloroplasts exhibit a fast and strong swelling after addition of dinactin (final concentration 10-5 m).

  2. 2.

    When illuminated the partieles shrink again and reach their original volume after 15 minutes. ATP may replace the light in this energy-dependent volume reduction.

  3. 3.

    Both swelling and subsequent shrinking are more pronounced in a hypertonic solution of KCl than in a NaCl-medium of the same concentration.

  4. 4.

    These results are discussed in relation to the known ion selectivity of the macrotetralides and the possible presence of light-driven K+ pump.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenoloxydase in Beta vulgaris. Plant Physiol. 24, 1 (1949).

    Google Scholar 

  • Bachofen, R., Specht-Jürgensen, I.: Die Wirkung von Dinactin auf die cyclische Photophosphorylierung, die lichtinduzierte ATP-P-Austauschreaktion und den lichtinduzierten Protonentransport in Chloroplasten. Planta (Berl.) 90, 66 (1970).

    Google Scholar 

  • Dilley, R. A., Vernon, L. P.: Ion and water transport processes related to the light-dependent shrinkage of spinach chloroplasts. Arch. Biochem. 111, 365 (1965).

    Google Scholar 

  • Eisenmann, G.: Ion permeation of cell membranes and its models. Fed. Proc. 27, 1249, 1968.

    Google Scholar 

  • Itoh, M., Izawa, S., Shibata, K.: Shrinkage of whole chloroplasts upon illumination. Biochem. biophys. Acta. (Amst.) 66, 319 (1963).

    Google Scholar 

  • Karlish, S. J. D., Shavit, N., Avron, M.: On the mechanism of uncoupling in chloroplasts by ion-permeability inducing agents. Europ. J. Biochem. 9, 291 (1969).

    Google Scholar 

  • Keller-Schierlein, W., Gerlach, H.: Makrotetralide. Fortschr. Chem. org. Naturstoffe 26, 161 (1968).

    Google Scholar 

  • Lardy, H.: Influence of antibiotics and cyclic polyethers on ion transport in mitochondria. Fed. Proc. 27, 1278 (1968).

    Google Scholar 

  • Müller, P., Rudin, D. O.: Development of K+−Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics. Biochem. biophys. Res. Commun. 26, 398 (1967).

    Google Scholar 

  • Neumann, J., Jagendorf, A. T.: Light-induced pH-changes related to phosphorylation. Arch. Biochem. 107, 109 (1964).

    Google Scholar 

  • Nobel, P. S.: A rapid technique for isolating chloroplasts with high rates of endogeneous photophosphorylation. Plant Physiol. 42, 1389 (1967).

    Google Scholar 

  • —: Light-induced changes in the ionic content of chloroplasts in Pisum sativum. Biochem. biophys. Acta (Amst.) 172, 134 (1969).

    Google Scholar 

  • Orth, G. M., Cornwell, D. G.: The particle volume distribution in purified cornseedling chloroplasts. Biochem. biophys. Acta (Amst.) 54, 389 (1961).

    Google Scholar 

  • Packer, L., Deamer, D. W.: Studies on the effect of light on chloroplast structure. In: Photophysiology, current topics, vol. III (A. C. Giese, ed.), p. 91. New York: Acad. Press 1968.

    Google Scholar 

  • Pedersen, C. J.: Ionic complexes of macrocyclic polyethers. Fed. Proc. 27, 1305 (1968).

    Google Scholar 

  • Pioda, L. A. R., Wachter, H. A., Dohner, R. E., Simon, W.: Komplexe von Nonactin and Monactin mit Natrium- und Kalium- und Ammonium-Ionen. Helv. chim. Acta 50, 1373 (1967).

    Google Scholar 

  • Plengvidhya, D., Burris, R. H.: Inhibitors of Photophosphorylation and photoreduction. Plant Physiol. 40, 997 (1965).

    Google Scholar 

  • Pressmann, B. C.: Ionophorous antibiotics as models for biological transport. Fed. Proc. 27, 1283 (1968).

    Google Scholar 

  • Ridley, S. M., Leech, R. M.: The surivval of chloroplasts in vitro: particle volume distribution patterns as a criterion for assessing the degree of integrity of isolated chloroplasts. Planta (Berl.) 83, 20 (1968).

    Google Scholar 

  • Stefanac, Z., Simon, W.: Ion specific electrochemical behaviour of macrotetralides in membranes. Mikrochem. J. 12, 125 (1967).

    Google Scholar 

  • Tolberg, A. B., Macey, R. I.: Osmotic behaviour of spinach chloroplasts. Biochem. biophys. Acta (Amst.) 109, 424 (1965).

    Google Scholar 

  • Tosteson, D. C.: Effect of macrocyclic compounds on the ionic permeability of artificial and natural membranes. Fed. Proc. 27, 1269 (1968).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Allgemeine Botanik der Universität Zürich, Zurich, Schweiz

    Elisabeth Bosshard-Heer & Reinhard Bachofen

Authors
  1. Elisabeth Bosshard-Heer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Reinhard Bachofen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bosshard-Heer, E., Bachofen, R. Die Wirkung von Dinactin auf Volumenänderungen ganzer Chloroplasten im Licht und im Dunkeln. Planta 91, 204–211 (1970). https://doi.org/10.1007/BF00385479

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00385479

Keywords

Search

Navigation