Skip to main content
SpringerLink
Log in

The action of nitrite on NADP reduction by intact spinach chloroplasts

  • Short Communication
  • Published:
Planta Aims and scope Submit manuscript

Summary

Isolated, intact spinach chloroplasts incubated in light in the presence of HCO -3 and NO -2 have the greater proportion of their NADP present in the reduced form. The steady state concentration of NADPH in light in these chloroplasts is significantly higher in the presence of NO2 than in its absence. These results invalidate earlier conclusions (Grant and Canvin, 1970) that NO -2 inhibits photosynthesis by preventing NADP reduction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ben-Hayyim, C., Gromet-Elhanon, Z., Avron, M.: A specific method for the determination of NADPH. Analyt. Biochem. 28, 6–12 (1969).

    Google Scholar 

  • Grant, B. R., Atkins, C. A., Canvin, D. T.: Intracellular location of nitrate and nitrite reductase in spinach and sunflower leaves. Planta (Berl.) 94, 60–72 (1970).

    Google Scholar 

  • Grant, B. R., Canvin, D. T.: The effect of nitrite on oxygen evolution and carbon dioxide assimilation and the reduction of nitrate and nitrite by intact chloroplasts. Planta (Berl.) 95, 227–246 (1970).

    Google Scholar 

  • Heber, V. W., Santarius, K. A.: Compartmentation and reduction of pyridine nucleotides in relation to photosynthesis. Biochim. biophys. Acta (Amst.) 109, 390–407 (1965).

    Google Scholar 

  • Hiller, R. G., Bassham, J. A.: Inhibition of CO2 fixation by nitrous acid. Biochim. biophys. Acta (Amst.) 109, 607–610 (1965).

    Google Scholar 

  • Whatley, F. R., Arnon, D. I.: Photosynthetic phosphorylation in plants. In: Methods in enzymology, S. R. Colowick, N. O. Kaplon, eds., vol. 6, p. 308–313. New York: Acad. Press 1963.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biology Department, Queen's University, Kingston, Ontario, Canada

    B. R. Grant, R. LaBelle & B. S. Mangat

Authors
  1. B. R. Grant
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. LaBelle
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. S. Mangat
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the National Research Council of Canada.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grant, B.R., LaBelle, R. & Mangat, B.S. The action of nitrite on NADP reduction by intact spinach chloroplasts. Planta 106, 181–184 (1972). https://doi.org/10.1007/BF00383999

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00383999

Keywords

Search

Navigation