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The activation of a neuralizing factor in the neural plate is correlated with its homoiogenetic-inducing activity

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Summary

Neural plates which are induced in the dorsal ectoderm of Triturus by the underlying mesoderm acquire, in turn, neural-inducing activity. This process is correlated with the appearance of neural-inducing activity in the microsomal fraction of the neural plate homogenate. The high-speed supernatant also acquires inducing activity after neural induction, but to a lesser extent. The experiments suggest that a masked neuralizing factor, which is already present in the ectoderm, is in part activated and exported from the inducing neural plate cells.

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References

  • Asashima M (1980) Inducing effects of the grey crescent region of early developmental stages of Ambystoma mexicanum. Wilhelm Roux's Arch 188:123–126

    Google Scholar 

  • Born J, Grunz H, Tiedemann H, Tiedemanm H (1980) Biological activity of the vegetalizing factor: Decrease after coupling to polysaccharide matrix and enzymatic recovery of active factor. Wilhelm Roux's Arch 189:47–56

    Google Scholar 

  • Born J, Hoppe P, Janeczek J, Tiedemann H, Tiedemann H (1986) Covalent coupling of neuralizing factors from Xenopus to Sepharose beads: no decrease of inducing activity. Cell Differ (in press)

  • Grunz H (1985) Effect of concanavalin A and vegetalizing factor on the outer and inner ectoderm layers of early gastrulae of Xenopus laevis after treatment with cytochalasin B. Cell Differ 16:83–92

    Google Scholar 

  • Holtfreter J (1934) Der Einfluß thermischer, mechanischer und chemischer Eingriffe auf die Induktionsfähigkeit von Tritonkeimteilen. Wilhelm Roux's Arch 132:225–306

    Google Scholar 

  • Holtfreter J (1947) Neural induction in explants which have passed through a sublethal cytolosis. J Exp Zool 106:197–222

    Google Scholar 

  • Janeczek J, John M, Born J, Tiedemann H, Tiedemann H (1984a) Inducing activity of subcellular fractions from amphibian embryos. Wilhelm Roux's Arch 193:1–12

    Google Scholar 

  • Janeczek J, Born J, John M, Scharschmidt M, Tiedemann H, Tiedemann H (1984b) Ribonucleoprotein particles from Xenopus laevis eggs and embryos. Eur J Biochem 140:257–264

    Google Scholar 

  • Janeczek J, Born J, Hoppe P, Schwarz W, Tiedemann H, Tiedemann H (1986a) Informative molecules and induction in early embryogenesis. In: Hayashi Y, Serrero G, Sato GH (eds) Cellular endocrinology. Alan R. Liss, New York (in press)

    Google Scholar 

  • Janeczek J, Tiedemann H, Tiedemann H (1986b) Ooplasmic determinants in amphibians. In: Slavkin HC (ed) New discoveries and technologies in developmental biology. Alan R. Liss, New York (in press)

    Google Scholar 

  • John M, Born J, Tiedemann H, Tiedemann H (1984) Activation of a neuralizing factor in amphibian ectoderm. Wilhelm Roux's Arch 193:13–18

    Google Scholar 

  • Mangold O (1923) Transplantationsversuche zur Frage der Spezifität und der Bildung der Keimblätter. Wilhelm Roux's Arch 100:198–301

    Google Scholar 

  • Mangold O (1929) Experimente zur Analyse der Determination und Induktion der Medullarplatte. Wilhelm Roux's Arch 117:587–694

    Google Scholar 

  • Mangold O, Spemann H (1927) Über die Induktion von Medullarplatte durch Medullarplatte im jüngeren Keim, ein Beispiel homoögenetischer oder assimilatorischer Induktion. Wilhelm Roux's Arch 111: 341–422

    Google Scholar 

  • Nakamura O, Takasaki H, Yamane H, Obayashi N, Kono S, Ohamoto H, Okumoto T (1970) Inductive activity of the organizer in the morula and blastula of Triturus pyrrhogaster. Proc Jpn Acad 46:700–705

    Google Scholar 

  • Raven CP, Kloos S (1945) Induction of medial and lateral pieces of the archenteron roof with special reference to the determination of the neural crest. Acta Neerl Morphol 4:348–362

    Google Scholar 

  • Spemann H, Mangold H (1924) Über Induktion von Embryonalanlagen durch Implantation artfremder Organisatoren. Wilhelm Roux's Arch 109:599–638

    Google Scholar 

  • Spemann H (1936) Experimentelle Beiträge zu einer Theorie der Entwicklung. Springer, Berlin

    Google Scholar 

  • Tiedemann-Waechter H (1960) Die Selbstdifferenzierungsfähigkeit medianer und lateraler Teile der Rumpfmedullarplatte bei Urodelen. Wilhelm Roux's Arch 152:303–338

    Google Scholar 

  • Tiedemann Hildegard (1986a) Test of embryonic inducing factors: Advantages and Disadvantages of different procedures. In: Hayashi Y, Serrero G, Sato GH (eds) Cellular Endocrinology. Alan R. Liss, New York (in press)

    Google Scholar 

  • Tiedemann Hildegard (1986b) On the molecular mechanism of neural induction: Neural differentiation of Triturus ectoderm exposed to Hepes buffer. Wilhelm Roux's Arch 195:399–402

    Google Scholar 

  • Tiedemann H, Born J, Tiedemann H (1967) Embryonale Induktion und Hemmung der Ribonukleinsäure-Synthese durch Actinomycin D. Z Naturforsch 22b: 649–659

    Google Scholar 

  • Waechter H (1953) Die Induktionsfähigkeit der Gehirnplatte bei Urodelen und ihr median-laterales Gefälle. Wilhelm Roux's Arch 146:201–274

    Google Scholar 

  • von Woellwarth C (1952) Die Induktionsstufen des Gehirns. Wilhelm Roux's Arch 145:582–668

    Google Scholar 

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Grunz, H., Born, J., Tiedemann, H. et al. The activation of a neuralizing factor in the neural plate is correlated with its homoiogenetic-inducing activity. Roux's Arch Dev Biol 195, 464–466 (1986). https://doi.org/10.1007/BF00375750

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  • DOI: https://doi.org/10.1007/BF00375750

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