Abstract
The activity of single L-type Ca2+ channels is rapidly lost (run-down) when contact between the membrane and cytosol is interrupted. We have now achieved the stabilization of cardiac Ca2+ channel activity of guinea-pig ventricular myocytes by using either cytosol or defined components added to excised patches. The endogenous protease inhibitor, calpastatin, together with nucleotides, ATP + GTP, was found to prevent rundown as effectively as cardiac cytosolic solution. These results suggest the involvement of proteolysis by calpain in run-down of channel activity and enable the study of cardiac Ca2+ channel regulation with free access to both sides of the membrane.
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Romanin, C., Grösswagen, P. & Schindler, H. Calpastatin and nucleotides stabilize cardiac calcium channel activity in excised patches. Pflügers Archiv 418, 86–92 (1991). https://doi.org/10.1007/BF00370456
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DOI: https://doi.org/10.1007/BF00370456