Abstract
The vaccinia virus (VV) E3L gene product functions as a dsRNA binding protein that is involved in conferring an interferon-resistant phenotype upon the virus. Studies with a vaccinia virus (VV) E3L- deletion mutant (vP1080) have also demonstrated that the E3L gene product is critical for productive replication on certain cell substrates. While E3L was found to be nonessential for replication in chick embryo fibroblasts (CEFs), virus specifically deleted of E3L was found to be replication deficient in Vero, HeLa, and murine L929 cells. Further, the temporal block in replication appears to differ in these cell systems, as evidenced by the observed timing of protein synthesis inhibition. In Vero cells infected with the VV E3L- mutant, there was no detectable protein synthesis after 2 hr post-infection, whereas in L929 cells normal protein patterns were observed even at late times post-infection. Expression of a heterologous dsRNA binding protein, the reovirus σ3 protein, by the E3L- mutant virus restored near wild-type growth characteristics, suggesting the critical nature for regulating dsRNA levels in VV-infected cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
WatsonJ.C., ChangH., and JacobsB.L., Virology 185, 206–216, 1991.
GillardS., SpehnerD., DrillienR., and KirnA., Proc Natl Acad Sci USA 83, 5573–5577, 1986.
PerkusM.E., GoebelS.J., DavisS.W., JohnsonG.P., LimbachK., NortonE.K., and PaolettiE., Virology 179, 276–286, 1990.
SpehnerD., GillardS., DrillienR., and KirnA.J., J Virol 62, 1297–1304, 1988.
BennettV., J Biol Chem 267, 8703–8706, 1992.
InkB.S., GilbertC.S., and EvanG.I., J Virol 69, 661–668, 1995.
YuwenH., CoxJ.H., YewdellJ.R., BenninkJ.R., and MossB., Virology 195, 732–744, 1993.
BeattieE., DenzlerK.L., TartagliaJ., PerkusM.E., PaolettiE., and JacobsB.L., J Virol 69, 499–505, 1994.
DaviesM.V., ChangH.-W., JacobsB.L., and KaufmanR.L., J Virol 67, 1688–1692, 1993.
Whitaker-DowlingP.A. and YoungnerJ.S. Virology 137, 171–181, 1984.
BeattieE., PaolettiE., and TartagliaJ., Virology 210, 254–263, 1995.
KaufmanR., Curr Opin Biotechnol 5, 550–557, 1994.
PerkusM.E., KauffmanE.B., TaylorJ., MercerS., SmithD., Van DerHoevenJ., and PaolettiE., J Tissue Cult Methods 15, 72–81, 1993.
TartagliaJ., PerkusM.E., TaylorJ., NortonE.K., AudonnetJ.-C., CoxW.I., DavisS.W., Van DerHoevenJ., MeignierB., RiviereM., LanguetB., and PaolettiE., Virology 188, 217–232, 1992.
DrillienR., SpehnerD., and KirnA., J Virol 28, 843–850, 1978.
Ramsey-EwingA. and MossB., Virology 206, 984–993, 1995.
HrubyD.E., LynnD.L., ConditR.C., and KatesJ.R., J Gen Virol 47, 485–488, 1980.
TaylorJ., MeignierB., TartagliaJ., LanguetB., Van DerHoevenJ., FranchiniG., TrimarchiC., and PaolettiE., Vaccine 13, 539–549, 1995.
PerkusM.E., LimbachK., and PaolettiE., J Virol 63, 3829–3836, 1989.
ParkH., DaviesM.V., LanglandJ.O., ChangH.-W., NamY.-S., TartagliaJ., PaolettiE., JacobsB.L., KaufmanR.J., and VenkatesanS., Proc Natl Acad Sci USA 91, 4713–4717, 1994.
ClemR.J., FechheimerM., and MillerL.K., Science 254, 1388–1390, 1991.
ShenY. and ShenkT., Proc Natl Acad Sci USA 91, 8940–8944, 1994.
TolskayaE.A., RomanovaL.I., KolesnikovaM.S., IvannikovaT.A., SmirnovaE.A., RaikhlinN.T., and AgolV.I., J Virol 69, 1181–1189, 1995.
LeeS.B. and EstebanM., Virology 193, 1037–1041, 1993.
BaylissC.D. and ConditR.C., Virology 194, 254–262, 1993.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beattie, E., Kauffman, E.B., Martinez, H. et al. Host-range restriction of vaccinia virus E3L-specific deletion mutants. Virus Genes 12, 89–94 (1996). https://doi.org/10.1007/BF00370005
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00370005