Skip to main content
SpringerLink
Log in

The fine structure of rat liver cells in suspension

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

The fine structure of liver cell suspensions prepared by two gentle methods of dispersal has been examined. Both methods result in extensive cell damage and in particular widespread rupture of the cell membrane. This damage does not occur in perfused liver slices. The observed damage to cell membranes could account for increased permeability. The limitations of such cell suspensions as models of biochemical function are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Berry, M. N.: Metabolic properties of cells isolated from adult mouse liver. J. Cell Biol. 15, 1–8 (1962).

    Google Scholar 

  • —, and F. O. Simpson: Fine structure of cells isolated from adult mouse liver. J. Cell Biol. 15, 9–17 (1962).

    Google Scholar 

  • Branster, M. V., and R. K. Morton: Isolation of intact liver cells. Nature (Lond.) 180, 1283–1284 (1957).

    Google Scholar 

  • Hayek, D. H., and S. R. Tipton: Respiratory activity and maintenance of cell suspensions of rat liver. J. Cell Biol. 29, 405–409 (1966).

    Google Scholar 

  • Iype, P. T., and P. M. Bhargava: The respiration of isolated hepatic cells in suspension. Biochem. J. 94, 284–288 (1965).

    Google Scholar 

  • Jacob, S. T., and P. M. Bhargava: A new method for the preparation of liver cell suspensions. Exp. Cell Res. 27, 453–467 (1962).

    Google Scholar 

  • — Metabolism of tissue cells in suspension. Synthesis of ribonucleic acid by liver cells in suspension. Biochem. J. 95, 568–576 (1965a).

    Google Scholar 

  • — Effect of chloramphenicol on R.N.A. synthesis in liver cells in suspension. Biochem. J. 97, 67–73 (1965b).

    Google Scholar 

  • Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).

    Google Scholar 

  • Shanmugan, G., and P. M. Bhargava: The uptake of homologous R.N.A. by rat liver parenchymal cells in suspension. Biochem. J. 99, 297–307 (1966).

    Google Scholar 

  • Zetterqvist, H.: The ultrastructural organisation of the columnar absorbing cells of the mouse jejunum. Stockholm: Aktiebolaget Godvil 1956.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Glasgow, Glasgow, Scotland

    Katharine E. Carr, J. P. Arbuthnott, P. G. Toner & C. G. Gemmell

  2. Department of Microbiology, University of Glasgow, Glasgow, Scotland

    Katharine E. Carr

Authors
  1. Katharine E. Carr
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. P. Arbuthnott
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. G. Toner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. G. Gemmell
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carr, K.E., Arbuthnott, J.P., Toner, P.G. et al. The fine structure of rat liver cells in suspension. Zeitschrift für Zellforschung 79, 265–271 (1967). https://doi.org/10.1007/BF00369290

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00369290

Keywords

Search

Navigation