Abstract
Cytotoxic effector T cells putatively specific for multiple non-H-2 histocompatibility (H) antigens were generated by immunizing and boosting C57BL/6 and B6.C-H-2 dmice with BALB.B and BALB/c stimulator cells, respectively. The generated effectors were tested for cell-mediated lympholysis on a panel of targets whose BALB/c-derived non-H-2 H antigens were donated by CXB recombinant inbred mice. The spectrum of reactivity of cytotoxic effector T cells with CXB targets demonstrated that the effectors did not recognize multiple H antigens but rather preferentially recognized a single immunodominant non-H-2 H antigen. The identity of the immunodominant H antigen was determined by the H-2 genotype of the stimulator cells when (B6 × B6.C-H-2 d)F 1 cytotoxic effectors were tested. These observations indicate that despite the fact that responders were challenged with more than 40 individual non-H-2 H antigens, they preferentially responded to a single immunodominant antigen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, D. W.: Recombinant inbred strains. Transplantation 11: 325–327, 1971a
Bailey, D. W.: Cumulative or independent effect. Transplantation 11: 419–422, 1971b
Bailey, D. W.: Genetics of histocompatibility in mice. I. New loci and congenic lines. Immunogenetics 2: 249–256, 1975
Bailey, D. W. and Mobraaten, L. E.: Estimates of the number of loci contributing to the histocompatibility between C57BL/6 and BALB/c strains of mice. Transplantation 7: 394–399, 1969
Bevan, M. J.: Interaction antigens detected by cytotoxic T cells with the major histocompatibility complex as modifier. Nature 256: 419–421, 1975a
Bevan, M.J.: The major histocompatibility complex determines susceptibility to cytotoxic T cells directed against minor histocompatibility antigens. J. Exp. Med. 142: 1349–1364, 1975b
Click, R. E., Benck, L., and Alter, B. J.: Immune responses in vitro. I. Culture conditions for antibody synthesis. Cell. Immunol. 3: 264, 1972
Fischer-Lindahl, K. and Hausmann, B.: Qed-1-a target for unrestricted killing by T cells. Eur. J. Immunol. 10: 289–298, 1980
Gordon, R. D., Simpson, E., and Samelson, L. E.: In vitro cell-mediated immune responses to the male specific (H-Y) antigen in mice. J. Exp. Med. 142: 1108–1120, 1975
Graff, R. J.: Polymorphism of histocompatibility genes in mice. Transplant. Proc. 1: 15–23, 1970
Graff, R. J., Silvers, W. K., Billingham, R. E., Hildenmann, W. H., and Snell, G. D.: The cumulative effect of histocompatibility antigens. Transplantation 4: 605–617, 1966
Johnson, L. L., Bailey, D. W., and Mobraaten, L. E.: Genetics of histocompatibility in mice. II. Survey for interactions between minor (non-H-2) antigens by skin grafting. Immunogenetics 11: 363–372, 1980
Johnson, L. L., Bailey, D. W., and Mobraaten, L. E.: Antigenic competition between minor (non-H-2) histocompatibility antigens. Immunogenetics 13: 451–456, 1981
Klein, J.: Transplantation immunity to antigens of the H-1 and H-3 locus: Strength, the effects of dosage, the additive effect, the development of H-3 antigens. Folia Biol. (Praha) 11: 169–175, 1965
McKhann, C. F.: Transplantation studies of strong and weak histocompatibility barriers in mice. I. Immunization. Transplantation 2: 613–619, 1964
Peck, A. B., and Bach, F. H.: A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media. J. Immunol. Methods 3: 147–154, 1973
Pfizenmaier, K., Pan, S., and Knowles, B. B.: Preferential H-2 association in cytotoxic T cell responses to SV40 tumor-associated specific antigens. J. Immunol. 124: 1888–1891, 1980
Schwartz, R. H., Yano, A., and Paul, W. E.: Interactions between antigen-presenting cells and primed T lymphocytes. An assessment of It gene expression in the antigen-presenting cell. Immunol. Rev. 40: 153–180, 1978
Simpson, E. and Gordon, R. D.: Responsiveness to H-Y antigen. Ir gene complementation and target cell specificity. Immunol. Rev. 35: 59–75, 1977
Wettstein, P. J. and Frelinger, J. A.: H-2 effects on cell-cell interactions in the response to single non-H-2 alloantigens. II. H-2 D region control of H-7.1 specific stimulator function in mixed lymphocyte culture and susceptibility to lysis by H-7.1 specific cytotoxic cells. J. Exp. Med. 146: 1356–1366, 1977
Wettstein, P. J. and Frelinger, J. A.: H-2 effects on cell-cell interactions in the response to single non-H-2 alloantigens. III. Evidence for a second Ir-gene system mapping in the H-2K and H-2D regions. Immunogenetics 10: 211–225, 1980
Wettstein, P. J., Bailey, D. W., Mobraaten, L. E., Klein, J., and Frelinger, J. A.: T lymphocyte response to H-2 mutants: Cytotoxic effectors are Ly-1+2+. Proc. Natl. Acad. Sci. U.S.A. 76: 3455–3459, 1979
Zaleski, M. and Klein, J.: Genetic control of the immune response to Thy-1 antigens. Immunol. Rev. 38: 120–162, 1978
Zinkernagel, R. M., Althage, A., Cooper, S., Kreels, G., Klein, P. A., Sefton, B., Flaherty, L., Stimpfling, J., Shreffler, D., and Klein, J.: Ir genes in H-2 regulate generation of anti-viral cytotoxic T cells. Mapping to K or D and dominance of responsiveness. J. Exp. Med. 148: 592–605, 1978
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wettstein, P.J., Bailey, D.W. Immunodominance in the immune response to “multiple” histocompatibility antigens. Immunogenetics 16, 47–58 (1982). https://doi.org/10.1007/BF00364441
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00364441