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Einfluß von Insulin auf das Membranpotential bei alimentärem Kaliummangel

Messungen am isolierten Rattenzwerchfell

Influence of insulin on membrane potentials in potassium deficiency

Measurements in isolated rat diaphragm

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Summary

In 102 single muscle cells of 7 rats with alimentary potassium depletion we found under influence of insulin (0.1 I.U./ml) a depolarisation of 11.2 mV, i.e. from −94.6 (s=±6.4)mV (100 cells) to −83.4 (s=±6.8)mV (p<0.001). The potassium concentration in the incubation medium was 4.7 (s=±0.29) mequ/l. — In addition we measured under influence of insulin (0.1 I.U./ml) an intracellular potassium concentration of 130 mval/lH2O IZR, which is probably higher than in potassium deficient animals without insulin (p<0.05). These findings suggest that insulin produces a netto potassium influx in potassium deficient animals, which could explain the depolarisation.

Zusammenfassung

An 102 Zellen des Zwerchfells von insgesamt 7 Ratten mit alimentärem Kaliummangel fanden wir unter dem Einfluß von Insulin (0,1 I.E./ml) eine Depolarisation um 11,2 mV, nämlich von −94,6 (s=±6,4) mV bei insgesamt 100 Zellen auf −83,4 (s=±6,8)mV (p < 0,001). Die Kaliumkonzentration in der Inkubationslösung betrug 4,7 (s=±0,29) mval/l. — Ferner steigt die bei kaliumverarmten Tieren erniedrigte intracelluläre Kaliumkonzentration unter Insulineinfluß von 107 (s=±12) mval/lH2O IZR auf 130 (s=±19,8) mval/lH2O IZR an (p<0,05). Die Befunde sprechen dafür, daß Insulin bei kaliumverarmten Tieren einen Netto-Kaliumeinstrom bewirkt, der eine Abnahme des Membranpotentials zur Folge hat.

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Mit Unterstützung der Deutschen Forschungsgemeinschaft.

Technische Assistenz: S. Winterling.

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Bolte, HD., Lüderitz, B. Einfluß von Insulin auf das Membranpotential bei alimentärem Kaliummangel. Pflügers Archiv 301, 254–258 (1968). https://doi.org/10.1007/BF00363772

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  • DOI: https://doi.org/10.1007/BF00363772

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