Skip to main content
SpringerLink
Log in

Accessibility of the minor groove of DNA in chromatin to the binding of antibiotics netropsin and distamycin A

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The interaction of the antibiotics distamycin A, distamycin analogue and netropsin with chromatin of calf thymus has been studied by circular dichroism measurements and by gel filtration. The minor groove of DNA in chromatin is accessible by 83–89% to the binding of these antibiotics as compared with that of free DNA. The present results combined with our data on the methylation of chromatin with dimethylsulphate [3] strongly suggest that the minor groove of DNA in chromatin is not occupied by chromatin proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DM:

distamycin A

DM2 :

analogue of distamycin

Nt:

netropsin

CD spectra:

circular dichroism spectra

References

  1. Simpson, R. T., Adv. in Enzymology 38, 41 (1973).

    Google Scholar 

  2. Simpson, R. T., Biochemistry 9, 4814 (1970).

    Google Scholar 

  3. Mirzabekov, A. D. and Melnikova, A. F., Mol. Biol. Reports 1, 385 (1974).

    Google Scholar 

  4. Mirzabekov, A. D. and Kolchinsky, A. M., Mol. Biol. Reports 1, 379 (1974).

    Google Scholar 

  5. Kolchinsky, A. M., Mirzabekov, A. D., Zasedatelev, A. S., Gursky, G. V., Grochovsky, S. L., Zhuse, A. L., and Gottikh, B. P., Mol. Biol. (U.S.S.R.) 9, 19 (1975).

    Google Scholar 

  6. Krey, A. K., Alison, R. G., and Hahn, F. E., FEBS Letters 29, 58 (1973).

    Google Scholar 

  7. Luck, G., Triebel, H., Waring, M. J., and Zimmer, Ch., Nucleic Acids Res. 1, 503 (1974).

    Google Scholar 

  8. Zasedatelev, A. S., Gursky, G. V., Zimmer, Ch., and Thrum, H., Mol. Biol. Reports 1, 337 (1974).

    Google Scholar 

  9. Wartell, R. M., Larson, J. E., and Wells, R. D., J. Biol. Chem. 249, 6719 (1974).

    Google Scholar 

  10. Zasedatelev, A. S., Gursky, G. V., Zimmer, Ch., and Thrum, H., Biochim. Biophys. Acta (submitted for publication).

  11. Dahmus, M. E. and McConnell, D. J., Biochemistry 8, 1524 (1969).

    Google Scholar 

  12. Marushige, J., and Bonner, J., J. Mol. Biol. 15, 160 (1966).

    Google Scholar 

  13. Burton, K., Biochem. J. 62, 315 (1956).

    Google Scholar 

  14. Gursky, G. V., Zasedatelev, A. S., Minyat, E. E., Ilyin, Yu. V., Georgiev, G. P., and Volkenstein, M. V., Mol. Biol. (U.S.S.R.) 8, 18 (1974).

    Google Scholar 

  15. Cerami, A., Reich, E., Ward, D. C., and Goldberg, I. H., Proc. Nat. Acad. Sci. U.S. 57, 1036 (1967).

    Google Scholar 

  16. Sobell, H. M., Jain, S. C., Sakore, T. D., and Nordman, C. E., Nature, New Biol. 231, 200 (1971).

    Google Scholar 

  17. Wang, J. C., J. Mol. Biol. 89, 783 (1974).

    Google Scholar 

  18. Zimmer, Ch., Luck, G., Thrum, H., and Pitra, C., Eur. J. Biochem. 26, 81 (1972).

    Google Scholar 

  19. Itzhaki, R. F., Biochem. J. 122, 583 (1971).

    Google Scholar 

  20. Clark, R. J. and Felsenfelf, G., Biochemistry 13, 3622 (1974).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the U.S.S.R., Moscow, USSR

    A. F. Melnikova, A. S. Zasedatelev, A. M. Kolchinsky, G. V. Gursky, A. L. Zhuze, S. L. Grochovsky & A. D. Mirzabekov

Authors
  1. A. F. Melnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. S. Zasedatelev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Kolchinsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. V. Gursky
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. L. Zhuze
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. L. Grochovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. D. Mirzabekov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Melnikova, A.F., Zasedatelev, A.S., Kolchinsky, A.M. et al. Accessibility of the minor groove of DNA in chromatin to the binding of antibiotics netropsin and distamycin A. Molecular Biology Reports 2, 135–142 (1975). https://doi.org/10.1007/BF00357544

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00357544

Keywords

Search

Navigation