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Relationship of nucleolus-associated bodies with the nucleolar organizer tracks in plant interphase nuclei (Pisum sativum)

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Abstract

Nucleolus-associated bodies (NABs) have long been noted in interphase nuclei of a wide variety of plant species. We have recently shown that these bodies consist largely of snRNPs and that they are located on the nucleolar surface in the immediate vicinity of the nucleolar organizer tracks. The present study revealed that, following exposure of roots to KCN, an agent that induces nucleolar segregation, NABs were intimately associated with intranucleolar chromatin. Although immunocytochemical tests with anti-DNA indicated that NABs contained no demonstrable amounts of DNA, our observations nevertheless add further support to the notion that these bodies are somehow related to the nucleolar chromosomes.

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References

  • Birnstiel ML, Hyde BB (1963) Protein synthesis by isolated pea nucleoli. J Cell Biol 18: 41–50

    Google Scholar 

  • Brasch K, Ochs RL (1992) Nuclear bodies (NBs): a newly “rediscovered” organelle. Exp Cell Res 202: 211–223

    Google Scholar 

  • Brown GL, Locke M (1978) Nucleoprotein localization by bismuth staining. Tissue Cell 10: 365–388

    Google Scholar 

  • Callan HG (1986) Lampbrush chromosomes, Springer, Berlin Heidelberg New York

    Google Scholar 

  • Carmo-Fonseca M, Pepperkok R, Carvalho MT, Lamond AI (1992) Transcription-dependent colocalization of the U1, U2, U4/U6 and U5 snRNPs in coiled bodies. J Cell Biol 117: 1–14

    Google Scholar 

  • Chamberland H, Lafontaine JG (1993) Localization of snRNPs antigens in nucleolus-associated bodies: study of plant interphase nuclei by confocal and electron microscopy. Chromosoma 102: 220–226

    Google Scholar 

  • Gall JG, Callan HG (1989) The sphere organelle contains small ribonucleoproteins. Proc Natl Acad Sci USA 86: 6635–6639

    Google Scholar 

  • Gosselin LA (1946) Les satellites chez les végétaux. Contributions de l'Institut d'Oka, no 2, pp 1–68

  • Halkka L (1981) Ultrastructural changes and kinetic relationships of the secondary nucleolus and nuclear bodies in previtellogenic oocyte of the dragonfly Cordulia aenea. Hereditas 95: 259–268

    Google Scholar 

  • Hardin JH, Spicer SS (1970) Ultrastructure of neuronal nucleoli of rat trigeminal ganglia: comparison of routine with pyroantimonate-osmium tetroxide fixation. J Ultrastruc Res 31: 16–36

    Google Scholar 

  • Hennig W (1987) The Y chromosomal lampbrush loops of Drosophila. In: Henning W (ed) Structure and function of eukaryotic chromosomes. Results and problems in cell differentiation 14. Springer, Berlin Heidelberg New York pp 133–146

    Google Scholar 

  • Hyde BH (1966) Changes in nucleolar ultrastructure associated with differentiation in the root tip. Natl Cancer Inst Monogr 23: 39–52

    Google Scholar 

  • Hyde BH (1967). Changes in nucleolar ultrastructure associated with differentiation in the root tip. J Ultrastruct. Res 18: 25–54

    Google Scholar 

  • Jensen AL, Brasch K (1985) Nuclear development in locust fat body: the influence of juvenile hormone on inclusion bodies and the nuclear matrix. Fissue Cell 17: 117–130

    Google Scholar 

  • Johnson DA, Gautsch JW, Sportsman JR, Elder JE (1984) Improved technique utilizing non fat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Anal Technol 1: 3–8

    Google Scholar 

  • Jordan EG (1984) Nucleolar Nomenclature. J Cell Sci 67: 217–220

    Google Scholar 

  • Kihlman BA (1966) Actions of chemicals on dividing cells. Prentice-Hall, Englewood Cliffs, USA

    Google Scholar 

  • Krainer AR (1988) Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 snRNPs. Nucleic Acids Res 16: 9415–9429

    Google Scholar 

  • Kuwada Y, Shinke N, Oura G (1938) Artificial uncoiling of the chromonema spirals as a method of investigation of the chromosome structure. Z Wiss Mikrosk Mikrosk Techn 55: 8–16

    Google Scholar 

  • Lafontaine JG (1965) A light and electron microscope study of small, spherical nuclear bodies in meristematic cells of Allium cepa, Vicia faba and Raphanus sativus. J Cell Biol 26: 1–17

    Google Scholar 

  • Lafontaine JG (1968) Structural components of the nucleus in mitotic plant cells. In: Dalton AJ, Haguenau F (eds) Ultrastructure in biological systems: the nucleus, vol 3. Academic Press, New York, pp 151–196

    Google Scholar 

  • Lafontaine JG, Luck BT, Gugg S (1991) Nucleolus-associated bodies in meristematic cells of two plant species (Cicer arietinum and Leucena glauca) with different ploidy levels. Can J Bot 69: 1329–1336

    Google Scholar 

  • Lerner MR, Boyle JA, Mount SM, Wolin SL, Steitz JA (1980) Are snRNPs involved in splicing? Nature 283: 220–224

    Google Scholar 

  • Levan A. (1949) The influence on chromosomes and mitosis of chemicals as studied by the Allium test. Proc Eight Intern Congr Genetics, Stockholm (Hereditas Suppl vol) pp 325–337

  • Luck BT, Lafontaine JG (1982) An ultracytochemical study of nuclear bodies in meristematic plant cells (Cicer arietinum). Can J Bot 60: 611–619

    Google Scholar 

  • Moreno Díaz de la Espina S, Franke WW, Krohne G, Trendelenburg CG, Scheer U (1982) Medusoid fibril bodies: a novel type of nuclear filament of diameter 8 to 12 nm with periodic ultrastructure demonstrated in oocytes of Xenopus laevis. Eur J Cell Biol 27: 141–150

    Google Scholar 

  • Moreno Díaz de la Espina S, Mínguez A, Vázquez-Nin GH, Echeverria OM (1992) Fine structural organization of a non-reticulate plant cell nucleus. An ultracytochemical and immunocytochemical study. Chromosoma 101: 311–321

    Google Scholar 

  • Moreno Díaz de la Espina S, Medina A, Mínguez A, Fernández-Gómez E (1993) Detection by bismuth staining of highly phosphorylated nucleo-proteins in plants. Determination of its specificity by X-ray microanalysis, SDS-PAGE and immunological analysis. Biol Cell (Paris) 77: 297–306

    Google Scholar 

  • Raska I, Ochs RL, Andrade LEC, Chan EKL, Burlingame R, Peebles C, Gruol D, Tan EM (1990) Association between the nucleolus and the coiled body. J Struct Biol 104: 120–127

    Google Scholar 

  • Risueño MC, Medina FJ (1982) Ultrastructural, cytochemical and autoradiographic characterization of coiled bodies in the plant cell nucleus. Biol Cell 44: 229–238

    Google Scholar 

  • Sankaranarayanan K, Hyde BB (1965) Ultrastructural studies of a nuclear body in peas with characteristics of both chromatin and nucleoli. J Ultrastruct Res 12: 748–761

    Google Scholar 

  • Thiry M (1992) Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogues. J Histochem Cytochem 40: 411–419

    Google Scholar 

  • Thomas P (1966) Etude, en microscopie électronique, de l'action de la pepsine et de la ribonucléase sur des cellules méristématiques de Radis et de Courge. C R Acad Sci (Paris), Ser 3. 262: 745–748

    Google Scholar 

  • Weber K, Rathke PC, Osborne M (1978) Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by fluorescence microscopy. Proc Natl Acad Sci USA 75: 1820–1824

    Google Scholar 

  • Wick SM, Hepler PK (1982) Selective localization of intracellular Ca2+ with potassium antimonate. J Histochem Cytochem 30: 1190–1204

    Google Scholar 

  • Williams LM, Jordan EG, Barlow PW (1983) The ultrastructure of nuclear bodies in interphase plant cell nuclei. Protoplasma 118: 85–103

    Google Scholar 

  • Williams LM, Charest PM, FitzGerald GJ, Lafontaine JG (1985) A comparison of nuclease-gold and protease-gold complex labelling over the nucleolus and nuclear bodies of Pisum sativum root tip cells. Biol Cell (Paris) 54: 65–72

    Google Scholar 

  • Wu Z, Murphy C, Callan HG, Gall JG (1991) Small nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes. J Cell Biol 113: 465–483

    Google Scholar 

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  1. Département de Biologie, Pavillon Marchand, Université Laval, G1K 7P4, Québec, Canada

    J. G. Lafontaine & H. Chamberland

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  1. J. G. Lafontaine
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  2. H. Chamberland
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Lafontaine, J.G., Chamberland, H. Relationship of nucleolus-associated bodies with the nucleolar organizer tracks in plant interphase nuclei (Pisum sativum). Chromosoma 103, 545–553 (1995). https://doi.org/10.1007/BF00355319

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  • DOI: https://doi.org/10.1007/BF00355319

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