Skip to main content
SpringerLink
Log in

A comparison of the ribosomal proteins of Drosophila ovary, adult, and embryo

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The proteins in the 80S ribosomes of Drosophila melanogaster ovaries and adults have been characterized by two-dimensional polyacrylamide gel electrophoresis. When ribosomal proteins of ovaries and adults were compared with those from embryos, all 3 tissues showed a similar number of proteins. In addition, qualitatively, the electrophoretograms of proteins extracted from the ribosomes of these 3 tissues were found to be indistinguishable. However, apparent quantitative differences in certain acidic proteins were observed between tissues. Using ribosomes from embryos as a standard for comparison, ribosomes from adult flies that were more than 14 days old appeared to have relatively larger amounts of acidic protiens S7 and S9, and relatively smaller amounts of acidic proteins S14 and S25/S27. The transition period occured during the ninth to thirteenth day of adult fly development. Significant differences were not detected between ovarian and embryonic acidic ribosomal proteins. In contrast to the differential ratio of acidic proteins in ovaries, adults, and embryos, a similar distribution of basic proteins was found in these tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bielke H, Welfe H, (1968) Characterization of ribosomal proteins from different tissues and species of animals by eletrophoresis on polyacrylamide gel. Mol Gen Genet 102:128–131

    Google Scholar 

  • Chooi WY (1980) Purification of Drosophila ribosomal proteins. Isolation of proteins S8, S13, S14, S16, S19, S20/L24, S22/L26, S24, S25/S27, S29, L4, L10/L11, L12, L13, L16, L18, L19, L27, 1, 7/8, 9 and 11. Biochemistry 19:3469–3476

    Google Scholar 

  • Chooi WY, Laird CD (1976) DNA and polyribosome-like structures in lysates of Drosophila melanogaster. J Mol Biol 100:493–518

    Google Scholar 

  • Chooi, WY, Sabatini LM, Macklin MD, Fraser D (1980) Group fractionation and determination of the number of ribosomal subunit proteins from Drosophila melanogaster embryos. Biochemistry 19:1425–1433

    Google Scholar 

  • Collatz E, Lin A, Stöffler G, Tsurugi K, Wool IG (1976) Group fractionation of eukaryotic ribosomal proteins. J Biol Chem 251:1808–1816

    Google Scholar 

  • Di Girolamo M, Cammarano P (1968) The protein composition of ribosomes and ribosomal subunits from animal tissues. Electrophoretic studies. Biochim Biophys Acta 168:181–194

    Google Scholar 

  • Fekete E, Lambertsson AG (1978) Imaginal disc ribosomal proteins in Drosophila melanogaster. Mol Gen Genet 159:85–87

    Google Scholar 

  • Houssais JF (1971) Nature de l'hétérogénéité interspécifique des protéines des ribosomes de mammifères. Eur J Biochem 24:232–241

    Google Scholar 

  • Kaltschmidt E, Wittmann HG (1970) Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for finger printing of ribosomal proteins. Anal Biochem 36:401–412

    Google Scholar 

  • Lambertsson AG, Rasmuson SB, Bloom GD (1970) The ribosomal proteins of Drosophila melanogaster. I. Characterization in polyacrylamide gel of proteins from larval, adult, and ammonium chloride-treated ribosomes. Mol Gen Genet 108:349–357

    Google Scholar 

  • Lambertsson AG (1972) The ribosomal proteins of Drosophila melanogaster. II. Comparison of protein patterns of ribosomes from larvae, pupae and adult flies by two-dimensional polyacrylamide gel electrophoresis. Mol Gen Genet 118:215–222

    Google Scholar 

  • Lambertsson AG (1975) The ribosomal proteins of Drosophila melanogaster IV. Characterization by two-dimensional gel electrophoresis of the ribosomal proteins from nine postembryonic developmental stages. Mol Gen Genet 139:133–144

    Google Scholar 

  • Low, RB, Wool IG (1967) Mammalian ribosomal protein: analysis by electrophoresis on polyacrylamide gel. Science 155:330–332

    Google Scholar 

  • Low RB, Wool IG, Martin TE (1969) Skeletal muscle ribosomal proteins: general characteristics and effect of diabetes. Biochim Biophys Acta 194:190–202

    Google Scholar 

  • MacInnes JW (1972) Differences between ribosomal subunits from brain and those from other tissues. J Mol Biol 65:157–161

    Google Scholar 

  • MacInnes JW (1973) Mammalian brain ribosomes are behaviourally and structurally heterogeneous. Nature New Biol 241:244–246

    Google Scholar 

  • McConkey, EH, Bielka H, Gordon J, Lastick SM, Lin A, Ogata K, Reboud J-P, Traugh JA, Traut RR, Warner JR, Welfe H, Wool IG (1979) Proposed uniform nomenclature for mammalian ribosomal proteins. Mol Gen Genet 169:1–6

    Google Scholar 

  • Mutolo V, Giudice G, Hoppe V, Donatuti G (1967) Species specificity of embryonic ribosomal proteins. Biochim Biophys Acta 138:214–217

    Google Scholar 

  • Sherton C, Wool IG (1974) A comparison of the proteins of rat skeletal muscle and liver ribosomes by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem 249:2258–2267

    Google Scholar 

  • Tsurugi K, Collatz E, Todokoro K, Ulbrich N, Lightfoot H, Wool IG (1978) Isolation of eukaryotic ribosomal proteins. J Biol Chem 253:946–955

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Indiana University, 47405, Bloomington, IN, USA

    W. Yean Chooi

Authors
  1. W. Yean Chooi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by K. Illmensee

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chooi, W.Y. A comparison of the ribosomal proteins of Drosophila ovary, adult, and embryo. Mol Gen Genet 184, 342–346 (1981). https://doi.org/10.1007/BF00352502

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00352502

Keywords

Search

Navigation