Abstract
Two glycolytic enzymes, phosphoglucose isomerase and fructose-1,6-bisphosphate aldolase, of Saccharomyces cerevisiae could be replaced by their heterologous counterparts from Escherichia coli and Drosophila melanogaster. Both heterologous enzymes, which show respectively little and no sequence homology to the corresponding yeast enzymes, fully restored wild-type properties when their genes were expressed in yeast deletion mutants. This result does not support notions of an obligatory formation of glycolytic multi-enzyme aggregates in yeast; nor does it support possible regulatory functions of yeast phosphoglucose isomerase.
Similar content being viewed by others
References
Aguilera A (1986) Mol Gen Genet 204:310–316
Aguilera A (1987) Curr Genet 11:429–434
Aguilera A Zimmermann FK (1986) Mol Gen Genet 202:83–89
Gergmeyer HU (1970) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim
Burke RL, Tekamp-Olson P, Najarian R (1983) J Biol Chem 258:2193–2201
Chaput M, Clacs V, Portetelle D, Cludts I, Cravador A, Burny A, Gras H, Tartar A (1988) Nature 332:454–455
Chiang HL, Schekman R (1991) Nature 350:313–318
Ciriacy M, Breitenbach I (1979) J Bacteriol 139:152–160
Entian KD (1986) Microbiol Sci 3:366–371
Faik P, Walker JIH, Redmill AAM, Morgan MJ (1988) Nature 332:455–456
François J, Van Schaftingen E, Hers HG (1984) Eur J Biochem 145:187–193
Froman BE, Tait RC, Gottlieb LD (1989) Mol Gen Genet 217:126–131
Gancedo C (1971) J Bacteriol 107:401–404
Gancedo JM (1992) Eur J Biochem 206:297–313
Gancedo JM Gancedo C (1979) Eur J Biochem 101:455–460
Gething MJ, Sambrook J (1992) Nature 355:33–45
Gietz RD, Sugino A (1988) Gene 74:527–534
Goffrini P, Wésolowski-Louvel M, Ferrero I (1991) Mol Gen Genet 228:401–409
Green JBA, Wright APH, Cheung WY, Lancashire WE, Hartley BS (1988) Mol Gen Genet 215:100–106
Guthrie C, Fink GR (eds) (1991) Methods Enzymol 194
Harris CE, Kobes RD, Teller DC, Rutter WJ (1969) Biochemistry 8:2442–2454
Hohmann S, Cederberg H (1990) Eur J Biochem 188:615–621
Holzer H (1984) Mechanism and function of reversible phosphorylation of fructose-1,6-bisphosphatase in yeast. In: Cohen P (ed) Molecular aspects of cellular regulation vol 3. Elsevier, Amsterdam, pp 143–154
Horecker BL, Tsolas O, Lai CY (1972) In: Boyer PD (ed) The enzymes, 3rd edn., vol 7. Academic Press, New York, pp 213–258
Kim J, Yim JJ, Wang S, Dorsett D (1992) Mol Cell Biol 12:773–783
Maitra PK, Lobo Z (1971) J Biol Chem 246:475–488
Marsh JJ, Lebherz HG (1992) Trends Biochem Sci 17:110–113
Moore PA, Sagliocco FA, Wood RMC, Brown AJP (1991) Mol Cell Biol 11:5330–5337
Ovádi J (1988) Trends Biochem Sci 13:486–490
Romanos MA, Scorer CA, Clare JJ (1992) Yeast 8:423–488
Rose MA, Entian KD, Hofmann L, Vogel RF, Mecke D (1988) FEBS Lett 241:55–59
Rose M, Albig W, Entian KD (1991) Eur J Biochem 199:511–518
Rothstein RJ (1983) Methods Enzymol 101:202–211
Saubrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schaaff I, Green JBA, Gozalbo D, Hohmann S (1989) Curr Genet 15:75–81
Schiestl RH, Gietz RD (1989) Curr Genet 16:339–346
Schmitt HD, Zimmermann FK (1982) J Bacteriol 151:1146–1152
Schmitt HD, Ciriacy M, Zimmermann FK (1983) Mol Gen Genet 192:247–252
Schwelberger HG, Kohlwein SD, Paltauf F (1989) Eur J Biochem 180:301–308
Shaw-Lee R, Lissemore JL, Sullivan DT, Tolan DR (1992) J Biol Chem 267:3959–3967
Srere PA (1987) Annu Rev Biochem 56:89–124
Thomas D, Cherest H, Surdin-Kerjan Y (1991) EMBO J 10:547–553
Zamenhoff S (1957) Methods Enzymol 3:696–704
Author information
Authors and Affiliations
Additional information
Communciated by K. Wolf
Rights and permissions
About this article
Cite this article
Boles, E., Zimmermann, F.K. Saccharomyces cerevisiae phosphoglucose isomerase and fructose bisphosphate aldolase can be replaced functionally by the corresponding enzymes of Escherichia coli and Drosophila melanogaster . Curr Genet 23, 187–191 (1993). https://doi.org/10.1007/BF00351494
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00351494