Abstract
Sympatric populations of two forms of the common dolphin, currently recognized collectively as Delphinus delphis Linnaeus, occur in several areas of the world's oceans. A molecular genetic study was initiated to determine whether these forms are genetically distinct in the Northeast Pacific. We compared mitochondrial DNA sequences from the control region and cytochrome b gene between specimens of the long-beaked and the short-beaked morphotypes collected between 1986 and 1989 off the coast of southern California. Additional short-beaked specimens collected from the eastern tropical Pacific (in 1978 and 1982) and the Black Sea (in 1989) were also compared. There were no shared mitochondrial DNA haplotypes between the two morphotypes, and both gene regions exhibited frequency and fixed nucleotide substitutions between the two morphotypes. This genetic differentiation, coupled with unique morphological characters of the short-beaked and long-beaked morphotypes determined in a parallel study, indicate that although sympatric, these populations of common dolphin are reproductively isolated from one another and may represent separate species.
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Anderson S, Bankier AT, Barrell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Sequence and organization of the human mitochondrial genome. Nature, Lond 290:457–465
Avise JC, Ball RM, Jr. (1990) Principles of genealogical concordance in species concepts and biological taxonomy. In: Futuyma D, Antonovics J (eds) Oxford surveys in evolutionary biology, Vol. 7. Oxford University Press, Oxford, pp 45–67
Baker SC, Palumbi SR, Lambertson RH, Weinrich MT, Calambokidis, J, O'Brien SJ (1990) Influence of seasonal migration on geographic distribution of mitochondrial DNA haplotypes in hump-back whales. Nature, Lond 344:238–240
Banks RC, Brownell RL (1969) Taxonomy of the common dolphins of the eastern Pacific Ocean. J Mammal 50:262–272
van Bree PJH, Purves PE (1972) Remarks on the validity of Delphinus bairdii (Cetacea, Delphinidae), J Mammal 53:372–374
Dall WH (1873) Preliminary description of three new species of Cetacea from the coast of California. Proc Calif Acad Sci 5:12–13
Davies JL (1963) The antitropical factor in cetacean speciation. Evolution, Lawrence, Kansas 17:107–116
Dizon AE, Southern SO, Perrin WF (1991) Molecular analysis of mtDNA types in exploited populations of spinner dolphins (Stenella longirostris). In: Hoelzel, AR (ed) Genetic ecology of whales and dolphins. Rep IWC, Special Issue 13, International Whaling Commission, Cambridge, pp 183–202
Felsenstein J (1988) PHYLIP. Phylogeny Inference Package. University of Washington Press, Seattle, Washington
Hershkovitz P (1966) Catalog of living whales US National Museum Bulletin 246. Smithsonian Institute, Washington, DC
Heyning JE, Perrin WF (1994) Two forms of common dolphins (genus Delphinus) from the eastern North Pacific; evidence for two species. Contr Sci (Los Angeles) 430:1–35
Hoelzel AR, Dover GA (1991) Genetic differentiation between sympatric killer whale populations. Heredity, Lond 66:191–195
Hoelzel AR, Hancock JM, Dover GA (1991) Evolution of the cetacean mitochondrial D-loop region. Molec Biol Evolut 8:475–493
Hultman T, Stahl S, Hornes E, Uhlen M (1989) Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res 17:4937–4945
Irwin DM, Kocher TD, Wilson AC (1991) Evolution of the cytochrome b gene of mammals. J molec Evolut 32:128–144
Kocher TD, Thomas WK, Meyer A, Edwards SV, Pääbo S, Villablanca FX, Wilson AC (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc natn Acad Sci USA 86:6196–6200
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Neigel JE, Avise JC (1986) Phylogenetic relationship of mitochondrial DNA under various demographic models of speciation. In: Karlin S, Nevo E (eds) Evolutionary processes and theory. Academic Press Inc., Orlando, pp 515–534
Palumbi S, Martin A, Romano S, McMillan WO, Stice L, Grabowski G (1991) The simples fool's guide to PCR. University of Hawaii, Honolulu
Perrin WF, Mitchell ED, Mead JG, Caldwell DK, Caldwell MC, van Bree PJH, Dawbin WH (1987) Revision of the spotted dolphins Stenella spp. Mar Mammal Sci 3:99–170
Perrin WF, Mitchell ED, Mead JG, Caldwell DK, van Bree PJH (1981) Stenella clymene, a rediscovered tropical dolphin of the Atlantic. J Mammal 62:583–598
Perrin WF, Mitchell ED, van Bree PJH (1978) Historical zoogeography of the tropical pelagic dolphins. II. In: Obrtel R, Folk Č, Pellantová J (eds) Congressus Theriologicus Internationalis. June 20–27, 1978. (Abstracts of papers). Brno, Czechoslovakia, pp 73
Schaeff C, Kraus S, Brown M, Perkins J, Payne R, Gaskin D, Boag P, White B (1991) Preliminary analysis of mitochondrial DNA variation within and between the right whale species Eubalaena glacialis and Eubalaena australis. In: Hoelzel AR (ed) Genetic ecology of whales and dolphins. Rept IWC, Special Issue 13, International Whaling Commission, Cambridge, pp 217–223
Southern S, Southern PJ, Dizon AE (1988) Molecular characterization of a cloned dolphin mitochondrial genome. J molec Evolut 28:32–42
Swofford DL (1989) PAUP. Phylogenetic analysis using parsimony. Computer program distributed by the Illinois Natural History Survey, Champaign, Illinois
Tajima F (1983) Evolutionary relationship of DNA sequences in finite populations. Genetics 105:437–460
Wada S, Kobayashi T, Numachi K (1991) Genetic variability and differentiation of mitochondrial DNA in minke whales. In: Hoelzel AR (ed) Genetic ecology of whales and dolphins. Rept IWC, Special Issue 13, International Whaling Commission, Cambridge, pp 203–215
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Communicated by J. P. Grassle, New Brunswick
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Rosel, P.E., Dizon, A.E. & Heyning, J.E. Genetic analysis of sympatric morphotypes of common dolphins (genus Delphinus). Marine Biology 119, 159–167 (1994). https://doi.org/10.1007/BF00349552
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DOI: https://doi.org/10.1007/BF00349552