Abstract
Two batches of experimental media containing 10% milk whey (sweet or sour) and 1% bovine serum supported the growth of BHK-21 cells in serial passages. The cell yield was greater than 2×106/ml at 48 h. The cells were susceptible to inoculation with foot and mouth disease (FMD) virus type ‘A’ and the virus titres obtained were comparable with those in Eagle's MEM-G with 10% serum. The use of milk whey could reduce the consumption of serum required for the growth of FMD virus in BHK-21 cells by up to 90%.
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Saha, S.N., Sen, A.K. Milk whey as a serum supplement for the growth of FMD virus in BHK-21 cells. Veterinary Research Communications 13, 89–92 (1989). https://doi.org/10.1007/BF00346718
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DOI: https://doi.org/10.1007/BF00346718