Abstract
A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 μg/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 μg/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.
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Abbreviations
- CRP:
-
C-reactive protein
- ELISA:
-
enzyme-linked immunosorbent assay
- RPLA:
-
reversed passive latex agglutination test
- SRID:
-
single radial immunodiffusion
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Tagata, K., Yokoyama, S., Ginbo, T. et al. Quantitative capillary reversed passive latex agglutination test for C-reactive protein (CRP) in the dog. Veterinary Research Communications 20, 21–30 (1996). https://doi.org/10.1007/BF00346573
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DOI: https://doi.org/10.1007/BF00346573