Summary
Allozymic variation in proteins encoded by 27 loci was analyzed electrophoretically in 218 adult specimens, mostly males, representing 8 populations, 5 central and 3 marginal, of lemon yellow treefrogs, Hyla arborea savignyi, in Israel along two transects of increasing aridity: (a) north to south and (b) west to east. The results indicate that (a) Of the 27 loci examined, 5 are monomorphic in all 8 populations; 9 are locally and weakly polymorphic; 6 are regionally and weakly polymorphic; and 7 are regionally and strongly polymorphic; (b) In the populations studied, no alternative fixations were found in any of the 27 loci, except in the Wasit population. The commonest allele predominates across all populations, except Wasit, central as well as marginal; (c) Clinal patterns associated with increasing aridity southwards and eastwards occur in polymorphism, P, heterozygosity, H, and in allele frequencies of Ldh-1, Fum, Got-1, and Sdh; (d) For habitat generalists, treefrogs have above average number of alleles per locus, A, and polymorphism, P, while the heterozygosity, H, is average. All three estimates A, P, and H exhibit wide geographic variation decreasing progressively southwards and eastwards; (e) Central populations harbor more genic variation then marginal populations; (f) Genic similarity between populations is high; (g) Significant gametic phase disequilibria were found in several tests in 2 populations; (h) P, H, and allozymic variation in several gene loci are significantly correlated and predictable by environmental variables, primarily those related to water; (i) Morphological and allozymic variations are uncorrelated.
The spatial patterns and environmental correlates and predictors of genic variation in Hyla arborea savignyi in Israel suggest that (i) protein polymorphisms are largely adaptive and are molded primarily by climatic selection rather than by stochastic processes or neutrality, and (ii) the environmental variation model seems to be the best predictor of genic variation in treefrogs.
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References
Babbel, G.R., Selander, R.K.: Genetic variability in edaphically restricted and widespread plant species. Evolution 28, 619–630. (1975)
Cavalli-Sforza, L.L.: Population structure and human evolution. Proc. R. Soc. London 164, 362–379 (1966)
Dessauer, H.C., Nevo, E., Chuang, K.C.: High genetic variability in an ecologically variable vertebrate, Bufo viridis. Biochem. Genet. 13, 651–661 (1975)
Dixon, W.J.: Biomedical computer programs. Univ. California Press, Berkeley. (1971)
Dobzhansky, Th.: Genetics and the origin of species. 3rd ed. New York: Columbia Univ. Press. (1951)
Draper, N.R., Smith, H.: Applied regression analysis. Wiley, N.Y. Chapters 3, 5, 6 (1966)
Estes, R., Reig, O.A.: The early fossil record of frogs: a review of the evidence. In: Evolutionary Biology of the Anurans. (Vial, J. ed.), Columbia Univ. of Missouri Press (1973)
Ewens, W.J., Feldman, M.W.: The theoretical asessment of selective neutrality. In: Population Genetics and Ecology. (S. Karlin and E. Nevo, eds.), pp. 303–338. New York: Academic Press (1976)
Ewens, W.J.: Population genetics theory in relation to the neutralist-selectionist controversy. Adv. Human Genet. 8, 67–134 (1977)
Gillespie, J.H., Kojima, K.: The degree of polymorphism in enzymes involved in energy production compared to that in nonspecific enzymes in two Drosophila ananassae populations. Proc. Nat. Acad. Sci. U.S.A. 61, 582–585 (1968)
Hedrick, P.W., Ginevan, M.E., Ewing, E.P.: Genetic polymorphism in heterogeneous environments. Ann. Rev. Ecol. Syst. 7, 1–32 (1976)
Inger, R.F., Voris, H.K., Voris, H.H.: Genetic variation and population ecology of some southeast Asian frogs of the genera Bufo and Rana. Biochem. Genet. 12, 121–145 (1974)
Johnson, G.B.: Enzyme polymorphism and metabolism. Science 184, 28–37 (1974)
Levene, H.: Genetic equilibrium when more than one ecological niche is available. Amer. Natur. 87, 331–333 (1953)
Levins, R.: Evolution in changing environments. Princeton, N.J.: Princeton Univ. Press (1968)
Lewontin, R.C., Krakauer, J.: Distribution of gene frequency as a test of the theory of the selective neutrality of polymorphisms. Genetics 74, 175–195 (1973)
Lewontin, R.C.: The Genetics Basis of Evolutionary Change. Columbia Univ Press. N.Y. (1974)
McDonald, J.F., Ayala, F.A.: Genetic response to environmental heterogeneity. Nature 250, 572–574 (1974)
McNab, B.K.: The metabolism of fossorial rodents: A study of convergence. Ecology 47, 712–733 (1966)
Mertens, R., Wermuth, H.: Die Amphibien und Reptilien Europas. Verlag Waldemar Kramer. Frankfurt am Main (1960)
Morrison, D.F.: Multivariate statistical methods. McGraw Hill, Inc. New York (1967)
Nei, M.: Genetic distance between populations. Amer. Natur. 106, 285–292 (1972)
Nevo, E.: Climatic adaptation in size of the green toad (Bufo viridis). Isr. J. Zool. (1972)
Nevo, E.: Genetic variation in constant environments. Experientia 32, 858–859 (1976b)
Nevo, E.: Genetic variation in natural populations: patterns and theory. Theor. Pop. Biol. 13, 121–177 (1978)
Nevo, E., Dessauer, H.C., Chuang, K.C.: Genetic variation as a test of natural selection. Proc. Nat. Acad. Sci. U.S.A. 72: 2145–2149 (1975)
Nevo, E., Shaw, C.R.: Genetic variation in a subterranean mammal, Spalax ehrenbergi. Biochem. Genet. 7, 235–241 (1972)
Nevo, E., Kim, J., Shaw, C.R., Thaeler, C. Jr.: Genetic variation, selection and speciation in Thomomys talpoides pocket gophers. Evolution 28, 1–23 (1974)
Nevo, E., Bar, Z.: Natural selection of genetic polymorphisms along climatic gradient. In: Population genetics and ecology (Karlin, S. and Nevo, E., eds.), Academic Press N.Y., pp. 159–184 (1976)
Nevo, E.: Adaptive strategies of genetic systems in constant and varying environments. In: Population Genetics and Ecology (S. Karlin and E. Nevo, eds.), pp. 141–158. Academic Press, 1976a
Nevo, E., Yang, S.Y.: Genetic structure and variation of green frog populations in Israel. (submitted) 1979
Patton, J.L., Yang, S.Y.: Genetic variation in Thomomys bottae pocket gophers: Macrogeographic patterns. Evolution 31, 697–720 (1977)
Powell, J.: Genetic polymorphisms in varied environments. Science 174, 1035–1036 (1971)
Schneider, H.: Rufe und Rufverhalten des Laubfrosches Hyla arborea arborea (L). Z. Vergl. Physiol. 57, 174–189 (1967)
Schneider, H.: Bio-akustische Untersuchungen am Mittelmeerlaubfrosch. Z. Vergl. Physiol. 61, 369–385 (1968)
Schneider, H., Nevo, E.: Bio-acoustic study of the yellow-lemon treefrog Hyla arborea savignyi. Audoin. Zool. Jb. Physiol. 76, 497–506 (1972)
Selander, R.K., Smith, M.H., Yang, S.Y., Johnson, W.E., Gentry, G.B.: Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse. (Peromyscus polionotus). Unv. Texas Publ. 7103, 49–90 (1971)
Somero, G.N., Soule', M.: Genetic variation in marine fishes as a test of the niche-variation hypothesis. Nature 249, 670–673 (1974)
Valentine, J.W., Ayala, F.J.: Genetic variability in krill. Proc. Nat. Acad. Sci. U.S.A. 73, 658–660 (1976)
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Nevo, E., Yang, S.Y. Genetic diversity and climatic determinants of tree frogs in Israel. Oecologia 41, 47–63 (1979). https://doi.org/10.1007/BF00344836
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DOI: https://doi.org/10.1007/BF00344836