Abstract
Rabbits were immunized with hybrids constructed with human and murine cells. Serological and immunochemical studies showed that xenoantiserum 1595 is operationally specific for HLA-A2, xenoantisera 0806 and 0746 for HLA-A3 and xenoantiserum 0745 for HLA-1327. The Fab2 blocking assay suggests a spatial relationship between allotypic determinants recognized by the xenoantisera and those reacting with the HLA-A, B-specific monoclonal antibodies (MoAb) Q1/28,6/3 1, Q6/64, and CR-1 and the anti-β 2-microglobulin (β 2-μ) MoAb NAMB-1.
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Aden, D. P., Mausner, R., and Knowles, B. B.: Production of HLA antibody in mice immunized with syngeneic mouse-human hybrid cells containing human chromosome 6. Nature 271: 375–377, 1978
Allison, J. P., Belvedere, M., Reisfeld, R. A., Pellegrino, M. A., and Ferrone, S.: Serological and immunochemical characterization of HLA-A9 xenoantisera. J. Immunol. 121: 579–585, 1978
Curry, R. A., Quaranta, V., Pellegrino, M. A., and Ferrone, S.: Serologically detectable human melanoma associated antigens are not genetically linked to HLA-A and B antigens. J. Immunol. 122: 2630–2632, 1979
Ferrone, S., Natali, P. G., Hunter, A., Terasaki, P. I., and Reisfeld, R. A.: Immunogenicity of soluble HLA alloantigens. J. Immunol. 108: 1718–1722, 1972
Ferrone, S., Pellegrino, M. A., Billing, R., Terasaki, P. I., and Reisfeld, R. A.: Production of anti-W24 xenoantisera in rabbits. Tissue Antigens 5: 41–47, 1975
Ferrone, S., Mittal, K. K., Pellegrino, M. A., Allison, J. P., and Reisfeld, R. A.: Immunogenicity of HLA antigens purified from serum. Transplantation 23: 7–15, 1977
Ferrone, S., Belvedere, M., and Pellegrino, M. A.: Structural relationship between W4/W6 antigens and other surface markers on cultured human lymphoid cells as determined by the “Lysostrip” method. Immunogenetics 6: 161–169, 1978
Grumet, F. C., Fendly, B., and Engleman, E. G.: Production and characterization of an anti-1327 hybridoma antibody (B27M1). Sixth Annual Meeting of the American Association of Clinical Histocompatibility Testing. American Association of Clinical Histocompatibility Testing, Kansas City Missouri, 1981 (Abstract A33)
Harris, R. and Zervas, J. D.: Reticulocyte HL-A antigens. Nature 221: 1062–1963, 1969
Imai, K., Galloway, D. R., and Ferrone, S.: Serological and immunochemical analysis of the specificity of the xenoantiserum 8986 elicited with hybrids between human melanoma cells and murine fibroblasts. Cancer Res., in press, 1981
Indiveri, F., Wilson, B. S., Pellegrino, M. A., and Ferrone, S.: Detection of human histocompatibility antigens with an indirect rosette microassay. J. Immunol. Methods 29: 101–109, 1979
Jones, C., Moore, E. E., and Lehman, D. W.: Genetic and biochemical analysis of the al cell-surface antigen associated with human chromosome 11. Proc. Natl. Acad. Sci. U.S.A. 76: 6491–6495, 1979
McCabe, R. P., Quaranta, V., Frugis, L., Ferrone, S., and Reisfeld, R. A.: A radioimmunometric antibody binding assay for the evaluation of xenoantisera to melanoma associated antigens. J. Natl. Cancer Inst. 62: 455–463, 1979
McConahey, P. J. and Dixon, F. J.: A method of trace iodination of proteins for immunologic studies. Int. Arch. Allergy Appl. Immunol. 29: 185–189, 1966
Nisonoff, A., Wissler, F. C., and Woernly, D. L.: Properties of univalent fragments of rabbit antibody isolated by specific adsorption. Arch. Biochem. Biophys. 88: 241–249, 1960
O'Farrell, P. H.: High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007–4021, 1974
Pellegrino, M. A., Ferrone, S., and Pellegrino, A.: A simple microabsorption technique for HL-A typing. Proc. Soc. Exp. Biol. Med. 139: 484–488, 1972
Pellegrino, M. A., Belvedere, M., Pellegrino, A. G., and Ferrone, S.: B peripheral lymphocytes express more HLA antigens than T peripheral lymphocytes. Transplantation 25: 93–95, 1978
Pellegrino, M. A., Richiardi, P., and Ferrone, S.: 8th International Histocompatibility Workshop: Analysis of a goat antiserum to HLA-1315 allospecificity. Tissue Antigens, in press, 1981
Quaranta, V., Pellegrino, M. A., and Ferrone, S.: Serologic and immunochemical characterization of the specificity of four monoclonal antibodies to distinct antigenic determinants expressed on subpopulations of human Ia-like antigens. J. Immunol. 126: 548–552, 1981a
Quaranta, V., Walker, L. E., Ruberto, G., Pellegrino, M. A., and Ferrone, S.: The free and the flzmicroglobulin-associated heavy chain of HLA-A, B alloantigens share the antigenic determinants recognized by the monoclonal antibody Q1/28. Immunogenetics 13: 285–295, 1981b
Reisfeld, R. A., Sevier, E. D., Pellegrino, M. A., Ferrone, S., and Poulik, M. D.: Association of HL-A antigens and β 2-microglobulin at the cellular and molecular level. Immunogenetics 2: 183–197, 1975
Richiardi, P., Pellegrino, M. A., and Ferrone, S.: Xenoantisera to allotypic specificities of HLA-A and B antigens. Transplantation 28: 333–338, 1979
Robb, R. J., Humphreys, R. E., Strominger, J. L., Fuller, T. C., and Mann, D. L.: Rabbit anti-HL-A2 sera. Transplantation 19: 445–447, 1975
Russo, C., Pellegrino, M. A., Ng, A.-K., Wilson, B. S., and Ferrone, S.: Antigenic constitution of HLAA, B, C molecules: Immunochemical analysis with monoclonal xenoantibodies. In S. Ferrone and B. Solheim (eds.): HLA Typing: Methodological Aspects and Relevance in Clinical Medicine. CRC Press, Boca Raton, in press, 1981
Strominger, J. L., Engelhard, V. H., Fuks, A., Guild, B. C., Hyafil, F., Kaufman, J. F., Korman, A. J., Kostyk, T. G., Krangel, M. S., Lancet, D., Lopez de Castro, J. A., Mann, D. L., Orr, H. T., Parham, P. R., Parker, K. C., Ploegh, H. L., Pober, J. S., Robb, R. J., and Shackelford, D. A.: Biochemical analysis of products of the MHC. In M. E. Dorf (ed.): The Role of the Major Histocompatibility Complex in Immunobiology, pp. 115–172, Garland STPM Press, New York and London, 1981
Wilson, B. S., Pellegrino, M. A., Reisfeld, R. A., and Ferrone, S.: A simple method for production of specific xenoantisera to human histocompatibility (HLA-A, B, C) antigens. Transplant. Proc. 10: 741–743, 1978
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Pellegrino, M.A., Ng, AK., Russo, C. et al. Immunochemical analysis of anti-HLA-A2, HLA-A3, and HLA-B27 xenoantisera elicited with hybrids between human and murine cells. Immunogenetics 14, 141–152 (1981). https://doi.org/10.1007/BF00344307
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DOI: https://doi.org/10.1007/BF00344307