Summary
The region of the reticular giganto-cellular nucleus, perfused with formalin and postfixed in osmium tetroxide, was studied with histochemical and electron microscopic techniques. The perikarya of the neurons have two zones. The peripheral cytoplasm contains Nissl bodies, mitochondria, and free RNP particles. The juxtanuclear cytoplasm contains the Golgi complex, mitochondria, RNP particles and dense bodies. The nucleus is indented and has a prominent nucleolus and a paranucleolar body. Dense bodies are found along the axon and dendrites as well. Three different types of synapses are described and two types of synaptic vesicles (spherical and ellipsoidal) are shown.
The capillary endothelium shows microvilli and marginal flaps. The endothelial cytoplasm contains vacuoles, micropinocytotic vesicles, and a few dense bodies. Processes of pericapillary cells, surrounded by a basement membrane, also contain dense bodies. The dense bodies found in the neurons and endothelial cells show acid phosphatase activity. On the basis of their morphology and their enzymatic activity these bodies are identified as lysosomes.
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References
Baker, J. R.: Cytological technique. London: Methuen & Co. 1946.
Barry, J., et G. Cotte: Étude microélectronique de l'éminence médiane du Cobaye. C. R. Soc. Biol. (Paris) 154, 2054–2055 (1960).
Bodian, D.: Introduction survey of neurons. Cold Spr. Harb. Symp. quant. Biol. 17, 1–14 (1952).
Cajal, S. R.: Histologie du système nerveux de l'homme et des vertébrés, (A. Maloine, éd.) Paris 1909–1911.
De Robertis, E.: The nucleo-cytoplasmic relationship and the basophilic substance (ergastoplasm) of nerve cell (electron microscopy observations). J. Histochem. Cytochem. 2, 341–345 (1954).
—: Histophysiology of synapses and neurosecretion, p. 16–69. London: Pergamon Press 1964.
—, and S. Bennett: Some features of the submicroscopic morphology of synapses in frog and earthworm. J. biophys. biochem. Cytol. 1, 47–58 (1955).
Duve, G. De, B. C. Pressman, R. Gianetto, R. Wattiaux, and F. Appelmans: Tissue fractionation studies. Intracellular distribution patterns of enzymes in rat liver tissue. Biochem. J. 60, 604–617 (1955).
Fawcett, D. W.: Comparative observations on the fine structure of blood capillaries. The peripheral blood vessels. International Academy of Pathology, Monograph, No 4, 17–44 (1963).
Follenius, E.: Étude comparative de la cytologie fine du noyau préoptique (NPO) et du noyau latéral du tuber (NLT) chez la truite (Salmo irideus Gibb) et chez la perche (Perca fluviatilis). Comparaison des deux types de neurosécrétion. Gen. comp. Endocr. 3, 66–85(1963).
Gabe, M.: Quelques applications de la coloration per la fuchsine-paraldehyde. Bull. Micr. appl. 3, 153–162 (1953).
Garcia, A., y E. M. Rodriguez: Presencia de material Gomori positivo en bulbo de la rata. Sesiones Científicas de Biología, Mendoza, Argentina, Resumen de Communicaciones, p. 40–41 (1960).
Gonzalez Aguilar, F., and E. de Robertis: A formalin perfusion fixation method for histophysiological study of the central nervous system with the electron microscope. Neurology (Minneap.) 13, 758–771 (1963).
Gray, E. G.: Axo-somatic and axo-dendritic synapses of the cerebral cortex; an electron microscope study. J. Anat. (Lond.) 93, 420–433 (1959).
Harnack, M. Von, and K. Lederis: Fine structure of the hypothalamo-neurohypophyseal secretory neurones. Gen. comp. Endocr. 2, 618–619 (1962).
Herndon, R. M.: The fine structure of the Purkinje cell. J. Cell Biol. 18, 167–180 (1963).
Holmes, R. L.: Comparative observations on inclusions in nerve fibres of the mammalian neurohypophysis. Z. Zellforsch. 64, 474–492 (1964).
Holt, S. J., and R. M. Hicks: The localization of acid phosphatase in rat liver cells as revealed by combined cytochemical staining and electron microscopy. J. biophys. biochem. Cytol. 11, 47–66 (1961).
Howe, A., and A. G. Pease: A histochemical investigations of neurosecretory substance in the rat. J. Histochem. Cytochem. 6, 561–569 (1956).
Hydén, H.: The neuron. In: The cell (J. Brächet and A. E. Mirsky, eds.), vol. IV, p. 215–323. New York and London: Academic Press 1960.
Kositzin, N. S.: Axo-dendritic relations in the brain stem reticular formation. J. comp. Neurol. 122, 9–17 (1964).
Luft, J. H.: The fine structure of the electric organ of the electric eel and torpedo ray. J. biophys. biochem. Cytol. 2, 229–231 (1956).
—: Improvements in epoxy resin embedding method. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Luse, S. A.: Electron microscopic observations of the central nervous system. J. biophys. biochem. Cytol. 2, 531–542 (1956).
Morato, M. J. X., et D. J. F. Ferreira: Recherches sur l'ultrastructure de l'area postrema. I. La paroi capillaire. Compt. Rend. Ass. Anat. 44e Réunion, Leyden, p. 541–548 (1957).
Murakami, M.: Elektronenmikroskopische Untersuchungen am Nucleus praeopticus der Kröte (Bufo vulgaris formosus). Z. Zellforsch. 63, 208–225 (1964).
Novikoff, A. B.: Lysosomes in the physiology and pathology of cells: contribution of staining methods. In: Ciba Foundation Symposium on Lysosomes p. 36–73 (A. V. S. De Reuck, and M. P. Cameron, eds.). Boston: Little Brown & Co. 1963.
Osinchak, J.: Electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activity in hypothalamic neurosecretory cells of the rat. J. Cell Biol. 21, 35–47 (1964).
Palay, S. L.: The fine structure of secretory neurons in the preoptic nucleus of the gold fish (Carassius auratus). Anat. Rec. 138, 417–443 (1960).
Pearse, A. F. E.: Histochemistry. Boston: Little Brown & Co. 1953.
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 47, 208–212 (1963).
Sabatini, D. D., K. Bensch, and R. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17, 19–58 (1963).
Schultz, R. L., E. A. Maynard, and D. C. Pease: Electron microscopy of neurons and neuroglia of the cerebral cortex and corpus callosum. Amer. J. Anat. 100, 369–388 (1957).
Stiennon, J. A., and P. Drochmans: Electron microscopy study of neurosecretory cells in Phasmidae. Gen. comp. Endocr. 1, 286–294 (1961).
Uchizono, K.: Characteristics of excitatory and inhibitory synapses in the central nervous system of the cat. Nature (Lond.) 207, 642–643 (1965).
Valverde, F.: Reticular formation of the pons and medulla oblongata. A Golgi study. J. comp. Neurol. 116, 71–99 (1961).
—: Reticular formation of the albino rat's brain stem. Cytoarchitecture and corticofugal connections. J. comp. Neurol. 119, 25–49 (1962).
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Partially supported by a school grant No RF 62051 from the Rockefeller Foundation, New York, USA, and Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.
Fellows of the Consejo National de Investigaciones Científicas y Técnicas, Argentina. The authors wish to thank Dr. Mario H. Burgos for his constant interest and criticism, and to Dr. Eduardo Rodriguez Echandia and Dr. Fabio L. Sacerdote for their valuable assistance.
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Santolaya, R.C., Rodriguez, E.M. The reticular substance of the medulla oblongata of the albino rat. Zeitschrift für Zellforschung 79, 537–549 (1967). https://doi.org/10.1007/BF00336311
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DOI: https://doi.org/10.1007/BF00336311