Summary
The c2 locus of Zea mays, identified as one of the genes affecting anthocyanin biosynthesis, was cloned using the transposable element En (Spm) as a gene tag. The Spm element present at the c2 locus in the autonomously mutating c2-m1 line was isolated using En1 element specific probes. Sequences flanking the element were identified as c2 locus specific and were used to clone the nonautonomous c2-m2 and wild-type alleles. The cloning and analysis of a cDNA complementary to the c2 locus provided evidence that this gene encodes the enzyme chalcone synthase.
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Communicated by P. Starlinger
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Wienand, U., Weydemann, U., Niesbach-Klösgen, U. et al. Molecular cloning of the c2 locus of Zea mays, the gene coding for chalcone synthase. Molec Gen Genet 203, 202–207 (1986). https://doi.org/10.1007/BF00333955
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DOI: https://doi.org/10.1007/BF00333955