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Interactions of Tn7 and temperate phage F116L of Pseudomonas aeruginosa

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Summary

Tn7 insertions into the genome of F116L, a Pseudomonas aeruginosa generalized transducing phage, were isolated by repeated cycles of transducing phage, were of strains lysogenic for F116cts mutants with selection for trimethoprim resistance (Tp1). Two non-defective F116Lcts:Tn7 phage were characterized. They have reduced plaquing ability, produced non-lysogenic Tpr transductants, and have yielded a deletion mutant of the phage genome upon selection for plaque formation in single infection. F116L DNA is circularly permuted and terminally redundant. A circular restriction map of 61.7 kb has been defined, and a cleavage site common to many enzymes has been identified at coordinate 23.3 kb on the map. It is presumed that this site represents the sequence for the initiation of DNA encapsidation by a headful packaging mode. The Tn7 insertion targets and a 13.4 kb deletion define regions of the F116L genome non-essential for either vegetative growth or lysogenization. The restriction map of Tn7 has been determined for five enzymes. Non-lysogenic Tpr transuctants reveal a Tn7 insertion hot-spot in the P. aeruginosa genome.

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Author notes

  1. M. Caruso

    Present address: Department of Pathology, New York University Medical Center, 10016, New York, New York, USA

Authors and Affiliations

  1. Department of Microbiology, University of Chicago, 920 East 58th Street, 60637, Chicago, Illinois, USA

    M. Caruso & J. A. Shapiro

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  1. M. Caruso
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  2. J. A. Shapiro
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Communicated by W. Arber

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Caruso, M., Shapiro, J.A. Interactions of Tn7 and temperate phage F116L of Pseudomonas aeruginosa . Mol Gen Genet 188, 292–298 (1982). https://doi.org/10.1007/BF00332690

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  • DOI: https://doi.org/10.1007/BF00332690

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