Summary
Mini-F sequences, including the promoter and portions of the ccd region, were inserted upstream of lacZ in promoterless lacZ vectors, and β-galactosidase specific activities were measured. The results showed that the H (ccdA), G (ccdB) and D genes, together with a promoter, comprise an operon. Ccd operon expression was shown to be regulated at the level of transcription by the G gene product, probably in concert with the H gene product. Thus expression is autoregulated. Expression of the D gene was largely dependent on the ccd promoter, although low levels of transcription from another promoter within the ccd coding region were detected.
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de Feyter, R., Wallace, C. & Lane, D. Autoregulation of the ccd operon in the F plasmid. Mol Gen Genet 218, 481–486 (1989). https://doi.org/10.1007/BF00332413
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DOI: https://doi.org/10.1007/BF00332413