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Structure and expression of the gene encoding the periplasmic arylsulfatase of Chlamydomonas reinhardtii

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Summary

Chlamydomonas reinhardtii produces a periplasmic arylsulfatase in response to sulfur deprivation. We have isolated and sequenced arylsulfatase cDNAs from a λ gt11 expression library. The amino acid sequence of the protein, as deduced from the nucleotide sequence, has features characteristic of secreted proteins, including a signal sequence and putative glycosylation sites. The gene has a broad codon usage with seven codons, all having A residues in the third position, not previously observed in C. reinhardtii genes. Arylsulfatase transcription is tightly regulated by sulfur availability. The ∼2.7 kb arylsulfatase transcript is very susceptible to degradation, disappearing in less than an hour after sulfur starved cells are administered either sulfate or α-amanitin. The accumulation of the arylsulfatase transcript is also suppressed by the addition of cycloheximide. Transcription initiation from the arylsulfatase gene occurs ∼ 100 bp upstream of the initiation codon, in a region that is 5′ to a 43 bp imperfect inverted repeat. Preceding the transcription start site are sequences similar to those present in promoter regions of other genes from C. reinhardtii.

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Author notes

  1. Eugenio L. de Hostos

    Present address: Abteilung Zellbiologie, Max Planck Institut für Biochemie, D-8033, Martinsried, Federal Republic of Germany

Authors and Affiliations

  1. Department of Biological Sciences, Stanford University, 94305, Stanford, CA, USA

    Eugenio L. de Hostos

  2. California Biotechnology, 2450 Bayshore Frontage Road, 94043, Mountain View, CA, USA

    James Schilling

  3. Department of Plant Biology, Carnegie Institution of Washington, 94305, Stanford, CA, USA

    Arthur R. Grossman

Authors
  1. Eugenio L. de Hostos
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  2. James Schilling
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  3. Arthur R. Grossman
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Communicated by E. Meyerowitz

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de Hostos, E.L., Schilling, J. & Grossman, A.R. Structure and expression of the gene encoding the periplasmic arylsulfatase of Chlamydomonas reinhardtii . Molec Gen Genet 218, 229–239 (1989). https://doi.org/10.1007/BF00331273

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