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Stability of ribosomal protein mRNA and translational feedback regulation in Escherichia coli

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Summary

It was previously observed that the stability of ribosomal protein (r-protein) mRNA in Escherichia coli decreases under the conditions where its translation is feedback inhibited by repressor r-protein. We have now demonstrated that the stability of mRNA for r-proteins S13, S11 and S4 increases in a strain carrying a mutation in the gene for S4, a translational repressor regulating these r-proteins. The results confirm the previous observations that translational repression increases the decay rate of r-protein mRNA, and in addition, show that the half-life of S13-S4 r-protein mRNA in cells growing under ordinary conditions is significantly shorter than its inherent stability would predict, due to the operation of translational feedback regulation.

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Author notes

  1. Phillip Singer

    Present address: La Jolla Cancer Research Foundation, 10901 N. Torrey Pines Road, 92037, La Jolla, CA, USA

  2. Masayasu Nomura

    Present address: Department of Biological Chemistry, California College of Medicine, University of California, Irvine, 91717, Irvine, CA, USA

Authors and Affiliations

  1. Institute for Enzyme Research and Departments of Genetics and Biochemistry, University of Wisconsin, 53706, Madison, WI, USA

    Phillip Singer & Masayasu Nomura

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  1. Phillip Singer
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  2. Masayasu Nomura
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Communicated by A. Böck

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Singer, P., Nomura, M. Stability of ribosomal protein mRNA and translational feedback regulation in Escherichia coli . Mol Gen Genet 199, 543–546 (1985). https://doi.org/10.1007/BF00330773

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  • DOI: https://doi.org/10.1007/BF00330773

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