Abstract
The binding of copper and zinc with substances contained in the rat bile was studied in the course of 24 h after intravenous administration of CuCl2 or ZnCl2. The bile was fractionated using column chromatography on Sephadex G-100 and in case of zinc also by the method of disc electrophoresis on polyacrylamide gel.
It was ascertained that both zinc and copper were bound at least with two different substances of bile differing by their molecular weight. The relation of copper content in the 1st and 2nd fractions changed in favour of the 1st fraction (macromolecular substances) in the course of 24 h. In the binding of zinc no significant changes were found in the course of 24 h after administration.
Zusammenfassung
Die Verfasser untersuchten im Verlauf von 24 Std nach intravenöser Verabreichung von CuCl2 oder ZnCl2 die Bindung von Kupfer und Zink an Substanzen, die in der Rattengalle enthalten sind. Zu diesem Zwecke fraktionierte man die Galle mit Hilfe der Säulenchromatographie auf Sephadex G-100 und im Falle von Zink auch mit Hilfe der Plattenelektrophorese auf dem Polyakrylamidgel.
Man stellte fest, daß sowohl Zink als auch Kupfer an wenigstens zwei verschiedene Gallenbestandteile gebunden sind, die sich durch ihr Molekulargewicht voneinander unterscheiden. Das Verhältnis vom Kupfergehalt in den 1. und 2. Fraktionen hat sich im Laufe von 24 Std zugunsten der ersten Fraktion (Makromolekularsubstanzen) geändert. In der Bindung von Zink hat man im Laufe von 24 Std nach dessen Verabreichung keine signifikanten Veränderungen gefunden.
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Cikrt, M., Havrdová, J. & Tichý, M. Changes in the binding of copper and zinc in the rat bile during 24 hours after application. Arch. Toxicol. 32, 321–329 (1974). https://doi.org/10.1007/BF00330114
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DOI: https://doi.org/10.1007/BF00330114