Skip to main content
SpringerLink
Log in

Conformational alterations in the proximal portion of the yeast invertase signal peptide do not block secretion

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Various amino acid insertions have been introduced into the proximal portion of the signal sequence of secreted yeast invertase. The altered invertase genes have been reintroduced into yeast and monitored for their ability to direct synthesis of secreted invertase in vivo. The insertions should alter the signal polypeptide local secondary structure as predicted by the Chou and Fasman rules (1978). Secretion of these altered invertase polypeptides is not blocked by the amino acid insertions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bostian KA, Lemire JM, Cannon LE, Halvorson HO (1980) In vitro synthesis of repressible yeast acid phosphatase: Identification of multiple mRNAs and products. Proc Natl Acad Sci USA 77:4504–4508

    Google Scholar 

  • Carlson M, Botstein D (1982) Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase. Cell 28:145–154

    Google Scholar 

  • Carlson M, Osmond BC, Botstein D (1981) Mutants of yeast defective in sucrose utilization. Genetics 98:25–40

    Google Scholar 

  • Carlson M, Taussig R, Kustu S, Botstein D (1983) The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence. Mol Cell Biol 3:439–447

    Google Scholar 

  • Chou PY, Fasman GD (1978) Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol 47:45–148

    Google Scholar 

  • Davis BD, Tai P-C (1980) The mechanism of protein secretion across membranes. Nature 283:433–438

    Google Scholar 

  • Emr SD, Bassford PJ (1982) Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations. J Biol Chem 257:5852–5860

    Google Scholar 

  • Emr SD, Silhavy TJ (1982) Molecular components of the signal sequence that function in the initiation of protein export. J Cell Biol 95:689–696

    Google Scholar 

  • Emr SD, Silhavy TJ (1983) Importance of secondary structure in the signal sequence for protein secretion. Proc Natl Acad Sci USA 80:4599–4603

    Google Scholar 

  • Emr SD, Hanley-Way S, Silhavy TJ (1981) Suppressor mutations that restore export of a protein with a defective signal sequence. Cell 23:79–88

    Google Scholar 

  • Engleman DM, Steitz TA (1981) The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis. Cell 23:411–422

    Google Scholar 

  • Esmon B, Novick P, Schekman R (1981) Compartmentalized assembly of oligosaccharides on exported glycoproteins in yeast. Cell 25:451–460

    Google Scholar 

  • Gascon S, Neumann NP, Lampen JO (1968) Comparative study of the properties of the purified internal and external invertases from yeast. J Biol Chem 243:1572–1577

    Google Scholar 

  • Goldstein A, Lampen JO (1975) β-D-fructofuranoside fructohydrolase in yeast. Methods in Enzymol 42:504–511

    Google Scholar 

  • Hopper JE, Bostian KA, Rowe LB, Tipper DJ (1977) Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae. J Biol Chem 252:9010–9017

    Google Scholar 

  • Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163–168

    Google Scholar 

  • James AA, Morrison PT, Kolodner R (1982) Genetic recombination of bacterial plasmid DNA: analysis of the effect of recombination deficient mutations on plasmid recombination. J Mol Biol 160:411–430

    Google Scholar 

  • Kreibich G, Czako-Graham M, Grebenau RC, Sabatini DD (1980) Functional and structural characteristics of endoplasmic reticulum proteins associated with ribosome binding sites. Ann NY Acad Sci 343:17–33

    Google Scholar 

  • Kreil G (1981) Transfer of proteins across membranes. Ann Rev Biochem 50:317–348

    Google Scholar 

  • Kumamoto C, Beckwith J (1983) Mutations in a new gene, secB, cause defective protein localization in Escherichia coli. J Bacteriol 154:253–260

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory. New York

    Google Scholar 

  • Messing J (1979) A multi-purpose cloning system based on the single-stranded DNA bacteriophage M13. Recombinant DNA Technical Bulletin, NIH Publication No. 79-99, 2, No. 2:449–453

  • Novick P, Ferro S, Schekman R (1981) Order of events in the yeast secretory pathway. Cell 25:461–469

    Google Scholar 

  • Oliver DB, Beckwith J (1981) E. coli mutant pleiotropically defective in the export of secreted proteins. Cell 25:765–772

    Google Scholar 

  • Perlman D, Halvorson HO (1981) Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene. Cell 25:525–536

    Google Scholar 

  • Perlman D, Halvorson HO (1983) A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J Mol Biol 167:391–409

    Google Scholar 

  • Perlman D, Halvorson HO, Cannon LE (1982) Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence. Proc Natl Acad Sci USA 79:781–785

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Sherman F, Fink G, Lawrence C (1977) Methods in yeast genetics. Cold Spring Harbor, New York

  • Shortle D, Nathans D (1978) Local mutagenesis: A method for generating viral mutants with base substitutions in preselected regions of the viral genome. Proc Natl Acad Sci USA 75:2170–2174

    Google Scholar 

  • Steiner DF, Quinn PS, Chan SJ, Marsh J, Tager HS (1980) Processing mechanisms in the biosynthesis of proteins. Ann NY Acad Sci 343:1–16

    Google Scholar 

  • Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) High frequency transformation of yeast: Autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci USA 76:1935–1939

    Google Scholar 

  • Taussig R, Carlson C (1983) Nucleotide sequence of the yeast SUC2 gene for invertase. Nucl Acids Res 11:1943–1954

    Google Scholar 

  • Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic primers. Gene 19:259–268

    Google Scholar 

  • von Heijne G (1981) On the hydrophobic nature of signal sequences. Eur J Biochem 116:419–422

    Google Scholar 

  • von Heijne G (1983) Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem 133:17–21

    Google Scholar 

  • Walter P, Blobel G (1982) Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum. Nature 299:691–698

    Google Scholar 

  • Walter P, Ibrahimi I, and Blobel G (1981) Translocation of proteins across the endoplasmic reticulum 1. Signal recognition protein (SRP) binds to in vitro-assembled polysomes synthesizing secretory protein. J Cell Biol 91:545–550

    Google Scholar 

  • Wickner W (1979) The assembly of proteins into biological membranes: the membrane trigger hypothesis. Ann Rev Biochem 48:23–45

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Rosenstiel Basic Medical Sciences Research Center and Department of Biology, Brandeis University, 02254, Waltham, MA, USA

    Patricia A. Brown, Harlyn O. Halvorson, Patricia Raney & Daniel Perlman

Authors
  1. Patricia A. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harlyn O. Halvorson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patricia Raney
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daniel Perlman
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by E. Bautz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brown, P.A., Halvorson, H.O., Raney, P. et al. Conformational alterations in the proximal portion of the yeast invertase signal peptide do not block secretion. Mol Gen Genet 197, 351–357 (1984). https://doi.org/10.1007/BF00329928

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00329928

Keywords

Search

Navigation