Summary
The modification in size and shape of chromosomes due to different technical procedures has been investigated on mammalian somatic chromosomes grown in tissue culture. Results show that (1) highly contracted chromosomes tend to have their centromeres more median than less contracted ones in different cells and (2) longer chromosomes tend to contract more strongly than shorter ones in the same cell by colchicine treatment. It was suggested that the process of chromatid condensation may not always be uniform in all chromosomes at a given stage of the mitotic cycle.
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Beatty, R.A.: How many chromosomes in mammalian somatic cells ? Int. Rev. Cytol. 3, 177–197 (1954).
Chu, E.H.Y., and H. Giles: Human chromosome complements in normal somatic cells in culture. Amer. J. hum. Genet. 11, 63–79 (1959).
Ford, C.E., and J.L. Hamerton: The chromosomes of man. Nature (Lond.) 178, 1020–1023 (1956).
—: Human somatic chromosomes. Nature (Lond.) 181, 1565–1568 (1958).
—: The chromosomes in a patient showing both mongolism and the Klinefelter syndrome. Lancet 1959 I, 709–710.
—, K. W. Jones, P.E. Polani, J.C. de Almedia and J.H. Briggs: A sex-chromosome anomaly in a case of gonadal dysgenesis (Turner's syndrome). Lancet 1959 I, 711–713.
— P. E. Polani, J. H. Briggs and P. M. F. Bishop: A presumptive human XXY/XX mosaic. Nature (Lond.) 183, 1030–1032 (1959).
Hsu, T. C., and C. M. Pomerat: Mammalian chromosomes in vitro, II. A method for spreading the chromosomes of cells in tissue culture. J. Hered. 44, 23–29 (1953).
Hungerford, D. A., A. J. Donnelly, P. C. Nowell and S. Beck: The chromosome constitution of a human phenotypic intersex. Amer. J. human. Genet. 11, 215–236 (1959).
Lejeune, J., R. Turpin et M. M. Gautier: Le mongolism premier exemple d'aberration autosomique humaine. Ann. Génét. 1, 41–49 (1959).
Levan, A., and T. C. Hsu: The human idiogram. Hereditas (Lund) 45, 665–674 (1959).
Makino, S., and M. Sasaki: On the chromosome number of man (A preliminary note). Proc. Jap. Acad. 35 99–104 (1959).
—: Chromosome constitution in normal human subjects and in one case of true hermaphroditism. (A preliminary note.) Proc. Jap. Acad. 36, 156–161 (1960).
- A chromosome survey in a Japanese population. Amer. J. hum. Genet. (in press).
McCoy, T. A., M. Maxwell and R. E. Neuman: The amino acid requirements of the Walker carcinoma 256 in vitro. Cancer Res. 16, 979–984 (1956).
Rothfels, K. H., and L. Siminovitch: An air-drying technique for flattening chromosomes in mammalian cells in vitro. Stain Technol. 33, 73–77 (1958).
Sasaki, M., and S. Makino: The chromosomal constitution of a human hermaphrodite. Tex. Rep. Biol. Med. 18, 493–500 (1960).
Tjio, J. H., and A. Levan: The chromosome number of man. Hereditas (Lund) 42, 1–6 (1956).
—, and T. T. Puck: The somatic chromosomes of man. Proc. nat. Acad. Sci. (Wash.) 44, 1229–1237 (1958).
— and A. Robinson: The somatic chromosomal constitution of some human subjects with genetic defects. Proc. nat. Acad. Sci. (Wash.) 45, 1008–1016 (1959).
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Contribution No. 471 from the Zoological Institute, Faculty of Science, Hokkaido University, Sapporo, Japan. Aided partly by a grant from the Rockefeller Foundation for research in cytology and partly by a grant from the Damon Runyon Memorial Fund for Cancer Research to S. Makino (DRG-563).
The author wishes to express his cordial thanks to Professor Sajiro Makino for his kind direction and improvement of the manuscript. Further appreciations should be extended to Drs. C.M. Pomerat and J. Blumel, University of Texas, who revised the data of this paper.
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Sasaki, M. Observations on the modification in size and shape of chromosomes due to technical procedure. Chromosoma 11, 514–522 (1960). https://doi.org/10.1007/BF00328672
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DOI: https://doi.org/10.1007/BF00328672