Abstract
We have analyzed different patent strains and gamma-ray induced mutants of Trichoderma harzianum by DNA fingerprinting and PCR fingerprinting (RAPD). Applying wild-type phage M13 DNA, with the oligonucleotides (CT)8 and (GTG)5 as probes for hybridization, as well as the oligonucleotides GGCATCGGCC, (GTG)5, (CAC)5 and the M13 sequence GAGGGTGGCGGTTCT as primers in PCR, we were able to obtain different and discriminative fingerprint patterns for all strains and mutants investigated. Irradiation of fungi led to mutations which resulted in new fingerprint patterns. Consequently, irradiation-induced mutants can be clearly distinguished from the original wild-type isolates by genomic fingerprinting which is of importance for the patent protection of fungal strains. Sequencing of the ITS-1 and ITS-2 regions of the rDNA gene complex revealed the same sequence for all mutant strains and the original wild-type strain.
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References
Ali S, Müller CR, Epplen JT (1986) Hum Genet 74:239–243
Braithwaite KS, Manners JM (1989) Curr Genet 16:473–475
Bruce A, King B (1986a) Material and Organismen 21:1–13
Bruce A, King B (1986b) Material and Organismen 21:165–179
Claydon N, Allan M, Hanson JR, Avent AG (1987) Trans Br Mycol Soc 88:503–513
Dunlop RW, Simon A, Sivasithamparam K, Ghisalberti E (1989) J Nat Products 52:67–74
Epplen JT (1988) J Hered 79:409–417
Gruber F, Visser J, Kubicek CP, De Graaff L (1990) Curr Genet 18: 71–76
Jeffreys AJ, Wislon V, Thein SL (1985) Nature 314:67–73
Lieckfeldt E, Meyer W, Kuhls K, Börner T (1992) Belg J Blot 125: 226–233
Meyer W, Koch A, Niemann C, Beyermann B, Epplen JT, Börner T (1991) Curr Genet 19:239–242
Meyer W, Morawetz R, Börner T, Kubicek CP, De Graaff L (1992a) Curr Genet 21:21–27
Meyer W, Lieckfeldt E, Wöstemeyer J, Börner T (1992b) Mycol Res 96:651–657
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Simon A, Dunlop R, Ghisalberi EL, Sivasithamparam K (1988) Soil Biol Biochem 20:263–264
Smith VL, Wilcox WF, Harman GE (1991) US-Patent 4, 996, 157
Speakman J, Scherer M, Anke H (1990) BRD-Patent DE 3904710 A1
Urbash I, Hansen PP (1990) 4th Int Mycol Cong, Regensburg, Poster IIE-260/3
Vassart G, Georges M, Monsieur R, Brocas H, Lequarre AS, Christophe D (1987) Science 235:683–684
Vigalys R, Hester M (1990) J Bacteriol 178:4238–4246
Weihe A, Niemann C, Lieckfeldt D, Meyer W, Börner T (1990) Fingerprint News 2:9–10
Welsh J, McClelland M (1990) Nucleic Acids Res 19:303–306
White TJ, Bruns TD, Lee SB, Taylor JW (1990) In: PCR Protocols, a guide to methods and applications. Academic Press, New York, pp 315–322
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) Nucleic Acids Res 18:6531–6535
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Communicated by R. J. Schweyen
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Schlick, A., Kuhls, K., Meyer, W. et al. Fingerprinting reveals gamma-ray induced mutations in fungal DNA: implications for identification of patent strains of Trichoderma harzianum . Curr Genet 26, 74–78 (1994). https://doi.org/10.1007/BF00326307
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DOI: https://doi.org/10.1007/BF00326307