Summary
Six chromosomal DNA bands of Schizophyllum commune have been resolved using transverse alternating field electrophoresis. The estimated sizes of the chromosomal DNAs ranged from 5.1 to 1.2 megabase pairs (Mb), the total genome size being approximately 35–36 Mb. Chromosomal length polymorphisms were found between the two S. commune isolates examined. The DNA bands corresponding to the two chromosomes containing the A and B mating-type loci were identified in hybridization experiments using probes specific to their respective linkage groups. The utility of eluted chromosomal DNAs as hybridization probes to select clones from genomic libraries, and the use of these clones in transformation experiments to identify genes of interest, are discussed.
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References
Brody H, Carbon J (1989) Proc Natl Acad Sci USA 86:6260–6263
Carle GF, Olson MV (1985) Proc Natl Acad Sci USA 82:3756–3760
Carmi P, Holm PB, Koltin Y, Rasmussen SW, Sage J, Zickler D (1978) Carlsberg Res Commun 43:117–132
Chang S-T, Wai C-C (1971) Genetics 68:13–19
Denhardt DT (1966) Biochem Biophys Res Commun 23:641–646
Dons JJM, De Vries OMH, Wessels JGH (1979) Biochem Biophys Acta 563:100–112
Fan J-B, Chikashige Y, Smith CL, Niwa O, Yanagida M, Cantor CR (1988) Nucleic Acids Res 17:2801–2818
Feinberg A, Vogelstein B (1983) Anal Biochem 132:6–13
Froeliger EH, Munoz-Rivas AM, Specht CA, Ullrich RC, Novotny CP (1987) Curr Genet 12:547–554
Froeliger EH, Ullrich RC, Novotny CP (1989) Gene 83:387–393
Giasson L (1990) Ph.D. Thesis, University of Vermont, Burlington, Vt., USA
Giasson L, Specht CA, Milgrim C, Novotny CP, Ullrich RC (1989) Mol Gen Genet 218:72–77
Lasker BA, Carle GF, Kobayashi GS, Medoff G (1989) Nucleic Acids Res 17:3783–3793
Magee BA, Koltin Y, Gorman JA, Magee PT (1988) Mol Cell Biol 8:4721–4726
Munoz-Rivas AM, Specht CA, Ullrich RC, Novotny CP (1986) Curr Genet 10:909–913
Orbach MJ, Vollrath D, Davis RW, Yanofsky C (1988) Mol Cell Biol 8:1469–1473
Raper CA (1988) In: Sidhu GS (ed) Advances in plant pathology vol 6. Academic Press, San Diego, pp 511–522
Raper CA (1990) In: O'Brien SJ (ed) Genetic maps. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 3.90–3.91
Raper JR, Hoffman RM (1974) In: King RC (ed) Handbook of genetics. Plenum Press, New York, pp 597–626
Raper JR, Boyd DH, Raper CA (1965) Proc Natl Acad Sci USA 53:1324–1332
Schwartz DC, Cantor CR (1984) Cell 37:67–75
Smith CL, Matsumoto T, Niwa O, Klco S, Fan F-B, Yanagida M, Cantor CR (1987) Nucleic Acids Res 15:4481–4489
Specht CA, Novotny CP, Ullrich RC (1984) Curr Genet 8:219–222
Stankis MM, Specht CA, Giasson L (1990) Sem Dev Biol 1:195–206
Timberlake WE (1978) Science 202:973–974
Ullrich RC, Droms KA, Doyon JD, Specht CA (1980) Exp Mycol 4:123–134
Ullrich RC, Novotny CP, Specht CA (1985) In: Moore D, Casselton LA, Wood DA, Frankland JC (eds) Developmental biology in higher fungi. Cambridge University Press, Cambridge, pp 467–484
Vollrath D, Davis RW (1987) Nucleic Acids Res 15:7865–7876
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Horton, J.S., Raper, C.A. Pulsed-field gel electrophoretic analysis of Schizophyllum commune chromosomal DNA. Curr Genet 19, 77–80 (1991). https://doi.org/10.1007/BF00326286
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DOI: https://doi.org/10.1007/BF00326286