Abstract
In the search for the mode of action of aluminum ions in dialysis encephalopathy, their interaction with calmodulin has been assessed, and compared with those of Ca2+, Pb2+, Mn2+, Hg2+ and Cd2+. The basal and calmodulin-dependent activity of phosphodiesterase was measured in the presence of the ions, and their binding to calmodulin was assessed by competition with 45Ca2+ in flow dialysis. Al3+, Mn2+, Hg2+, and Cd2+ cannot be regarded as exclusive calmodulin antagonists or agonists, but rather interact with the phosphodiesterase itself. Pb2+ however, mimicks Ca2+ in every system tested, and is active in the same concentration range as Ca2+. Our results indicate that the assumed role of Al3+ ions in dialysis encephalopathy or other neurological disturbances is not linked with calmodulin.
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Abbreviations
- EGTA:
-
Ethyleneglycol-bis-(β-aminoethyl ether)
- N,N,N′,N′:
-
tetraacetic acid
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Supported by a DFG fellowship
Part of the thesis of G. Federolf (FB 23, University of Giessen, FRG)
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Richardt, G., Federolf, G. & Habermann, E. The interaction of aluminum and other metal ions with calcium-calmodulin-dependent phosphodiesterase. Arch Toxicol 57, 257–259 (1985). https://doi.org/10.1007/BF00324788
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DOI: https://doi.org/10.1007/BF00324788